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Rat Anti-Mouse CD49d-LE/AF

1520-14
SouthernBiotech
ApplicationsFunctional Assay, Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
Product group Antibodies
TargetITGA4
Price on request
0.5 mg
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    SouthernBiotech
  • Product Name
    Rat Anti-Mouse CD49d-LE/AF
  • Delivery Days Customer
    7
  • Applications
    Functional Assay, Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    PS/2
  • Gene ID3676
  • Target name
    ITGA4
  • Target description
    integrin subunit alpha 4
  • Target synonyms
    269C wild type; alpha 4 subunit of VLA-4 receptor; antigen CD49D, alpha-4 subunit of VLA-4 receptor; CD49 antigen-like family member D; CD49D; IA4; integrin alpha-4; integrin alpha-IV; very late activation protein 4 receptor, alpha 4 subunit; VLA-4 subunit alpha
  • Host
    Rat
  • Isotype
    IgG2b
  • Protein IDP13612
  • Protein Name
    Integrin alpha-4
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin alpha 4 using anti-Integrin alpha 4 antibody (PB9648). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Kidney Tissue Lysate at 50ug, Lane 3: JURKAT Whole Cell Lysate at 40ug, Lane 4: 22RV1 Whole Cell Lysate at 40ug, Lane 5: HEPG2 Whole Cell Lysate at 40ug, Lane 6: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin alpha 4 antigen affinity purified polyclonal antibody (Catalog # PB9648) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin alpha 4 at approximately 220 kDa. The expected band size for Integrin alpha 4 is at 115 kDa.
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