Rat CTRC protein, His tag

GeneTex

Rat CTRC protein (active)

9

Elastase 4 (ELA4) is a member of the peptidase S1 family. The encoded protein is a serum calcium-decreasing factor that has chymotrypsin-like protease activity. It regulates activation and degradation of trypsinogens and procarboxypeptidases by targeting specific cleavage sites within their zymogen precursors. Has chymotrypsin-type protease activity and hypocalcemic activity. Besides, Plasminogen Activator, Urokinase Receptor (uPAR) has been identified as an interactor of ELA4, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat ELA4 and recombinant rat uPAR. Briefly, ELA4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 microl were then transferred to uPAR-coated microtiter wells and incubated for 2h at 37C. Wells were washed with PBST and incubated for 1h with anti-ELA4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37C. Finally, add 50 microl stop solution to the wells and read at 450nm immediately. The binding activity of of ELA4 and uPAR was in a dose dependent manner.

Functional Assay

Research Use Only

Unconjugated

Lyophilized

Chymotrypsin-C

-20°C or -80°C,2°C to 8°C

12352204