Rat Osteopontin protein, His tag (active)

GeneTex

Rat Osteopontin protein (active)

9

Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1beta which promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1x105 cells/ml, and treated with or without various concentrations of OPN for 24h and MCP-1 levels in the cell supernatant were determined by ELISA. MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN.

Functional Assay

Research Use Only

Unconjugated

Osteopontin

sialoprotein that binds osteosarcoma cells; may play a role in osteoclast cell function [RGD, Feb 2006]

-20°C or -80°C,2°C to 8°C

12352202