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RbAp48 + RbAp46 antibody [15G12]

GTX70236
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityHuman, Mouse
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Overview

  • Supplier
    GeneTex
  • Product Name
    RbAp48 + RbAp46 antibody [15G12]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 0.2 - 1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    15G12
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    RbAp48-15G12 recognizes full length RbAp48 (48 kDa) and RbAp46 (46 kDa), related nuclear proteins first isolated by their ability to interact with the carboxyl-terminus of the retinoblastoma protein (Rb). These two proteins are highly homologous at the amino acid level and are related functionally to members of the WD (Trp-Asp) repeat family of proteins. RbAp48 is one of the three subunits of CAF-1, can bind to histone H4 and is a subunit of human histone deacetylase HD1. Both RbAp48 and RbAp46 interact with HDAC1 and mSin3 in vivo.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • A phosphomimetic mutation in the Sall1 repression motif disrupts recruitment of the nucleosome remodeling and deacetylase complex and repression of Gbx2. Lauberth SM et al., 2007 Nov 30, J Biol Chem
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  • A conserved 12-amino acid motif in Sall1 recruits the nucleosome remodeling and deacetylase corepressor complex. Lauberth SM et al., 2006 Aug 18, J Biol Chem
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  • Murine Sall1 represses transcription by recruiting a histone deacetylase complex. Kiefer SM et al., 2002 Apr 26, J Biol Chem
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  • RBP1 recruits the mSIN3-histone deacetylase complex to the pocket of retinoblastoma tumor suppressor family proteins found in limited discrete regions of the nucleus at growth arrest. Lai A et al., 2001 Apr, Mol Cell Biol
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  • Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1. Humphrey GW et al., 2001 Mar 2, J Biol Chem
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  • An ikaros-containing chromatin-remodeling complex in adult-type erythroid cells. ONeill DW et al., 2000 Oct, Mol Cell Biol
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  • Histone binding protein RbAp48 interacts with a complex of CREB binding protein and phosphorylated CREB. Zhang Q et al., 2000 Jul, Mol Cell Biol
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  • BRCA1 interacts with components of the histone deacetylase complex. Yarden RI et al., 1999 Apr 27, Proc Natl Acad Sci U S A
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  • Tissue-specific and developmental stage-specific DNA binding by a mammalian SWI/SNF complex associated with human fetal-to-adult globin gene switching. ONeill D et al., 1999 Jan 19, Proc Natl Acad Sci U S A
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