Recombinant Mouse CCL3

Biorbyt

Recombinant Mouse CCL3

16

1. Reconstitute each vial of Nigericin with 100 microL DMSO to form the 5 mM stock concentrate. Once reconstituted, it may be aliquoted and stored at ≤ -20°C for 1 year protected from light and thawed no more than twice during that time., 2. Immediately prior to addition to the samples and controls, dilute 5 mM Nigericin stock 1:10 in diH2O to form a 500 microM working solution for use in treating samples. For example, dilute 1:10 by adding 20 microL stock concentrate to 180 microL diH2O., 3. Use Nigericin at 1-20 microM to induce NLRP3 inflammasome activation in cells. For example, to use at 10 microM, dilute 500 microM working solution 1:50 in samples; e.g., spike 294 microL cell suspension/overlay medium with 6 microL of 500 microM working solution. Typical treatment periods range from 3-24 hours at 37°C. Each investigator should adjust the concentration of Nigericin and treatment period to accommodate the particular cell line and research conditions., 4. NLRP3 inflammasome activation can be detected by ELISA or Western blot measuring secreted pro-inflammatory cytokines IL-1beta or IL-18, or through the use of caspase-1 activation assays, such as ICTs Caspase-1 Assay Kits or Pyroptosis/Caspase-1 Assay Kit.

Research Use Only

Chemokine ligand 3 (CCL3), also known as Macrophage inflammatory protein-1 alpha (MIP-1 alpha) is a small cytokine belonging to the CC chemokine family. CCL3/MIP-1 alpha is a chemoattractant for several different leukocytes, with varying degrees of potency (monocytes = T-cells>neutrophils>eosinophils).

-20°C

12352202