RFP antibody

Biorbyt

RFP antibody

16

Anti-RFP antibody has been tested by ELISA, SDS-Page, and Western blot and is designed to detect Red Fluorescent Protein and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

ELISA: 1:75,000 - 1:150,000, FC: User Optimized, IF: User Optimized, IP: User Optimized, WB: 1:1,000 - 1:10,000 ELISA, FC, IF, IP, WB

Research Use Only

Monoclonal

8E5.G7

Unconjugated

Liquid

Mouse

IgG2a

Red fluorescent protein drFP583

RFP antibody

Other Species

-20°C,2°C to 8°C

12352203