RFP antibody

Biorbyt

RFP antibody

16

Polyclonal anti-RFP is designed to detect RFP and its variants. This antibody has been tested by ELISA, western blot, IF, and IHC, and is suitable for use in IP, ICC, dual RNA-FISH, iDISCO+, IEM, and FLOW. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP (code # S000-03). Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.

Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry

ELISA: 1:20,000 - 1:50,000, FC: 1:200 - 1:2,000, IHC: 1:200-1:2,000, IF: 1:200-1:2,000, WB: 1:1,000 - 1:5,000 ELISA, FC, IF, IHC, WB

Research Use Only

Polyclonal

1.05 mg/ml

Unconjugated

Liquid

Rabbit

IgG

Red fluorescent protein drFP583

RFP antibody

Other Species

-20°C,See manual

12352203