1:1000 (1microg/mL) Ab dilution probed against HEK293 cells transfected with RFP-tagged protein vector; untransfected control (1), transfected with Turbo-RFP (2), and transfected with DsRed (3)
1:1000 (1microg/mL) Ab dilution probed against HEK293 cells transfected with RFP-tagged protein vector; untransfected control (1), transfected with Turbo-RFP (2), and transfected with DsRed (3)
RFP antibody [RF5R]
GTX82561
ApplicationsDot Blot, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
Product group Antibodies
ReactivityOther Species
Overview
- SupplierGeneTex
- Product NameRFP antibody [RF5R]
- Delivery Days Customer9
- Antibody SpecificityRecognizes native and denatured forms of RFP and its variants: TagRFP, TurboRFP, dsRed, mCherry, mOrange, tdTomato, etc.
- Application Supplier NoteRecommended Starting Dilutions:WB (with ECL): Use at a dilution of 1:1000-3000 Immunostaining: Use at a dilution of 1:500 - 1:2000For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
- ApplicationsDot Blot, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDRF5R
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- Scientific Descriptionince the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. RFP is the recent discovered protein that has the similar function and applications as GFP. Either GFP or RFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP or RFP can fuse with proteins of interest without interfering significantly with their assembly and function.
- ReactivityOther Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- A subset of gut leukocytes has telomerase-dependent hyper-long telomeres and require telomerase for function in zebrafish.Read more
- Is zebrafish heart regeneration complete? Lineage-restricted cardiomyocytes proliferate to pre-injury numbers but some fail to differentiate in fibrotic hearts. Bertozzi A et al., 2021 Mar, Dev BiolRead more
- Analysis of Spo0M function in Bacillus subtilis. Vega-Cabrera LA et al., 2017, PLoS OneRead more
- LIG4 mediates Wnt signalling-induced radioresistance. Jun S et al., 2016 Mar 24, Nat CommunRead more