ChIP analysis of sheared chromatin from 2x10? NALM cells using GTX60368 RFXAP antibody - ChIP grade. An anti-flag antibody (lane 2) was used as negative IP control. This figure shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel). Antibody amount : 5-7microg
ChIP analysis of sheared chromatin from 2x10? NALM cells using GTX60368 RFXAP antibody - ChIP grade. An anti-flag antibody (lane 2) was used as negative IP control. This figure shows the result of the end-point PCR with primers for HLA_DRA, used as positive control (lower panel) and for HLA_DOB, used a negative PCR control (upper panel). Antibody amount : 5-7microg
RFXAP antibody - ChIP grade
GTX60368
ApplicationsChIP Chromatin ImmunoPrecipitation
Product group Antibodies
ReactivityHuman
TargetRFXAP
Overview
- SupplierGeneTex
- Product NameRFXAP antibody - ChIP grade
- Delivery Days Customer9
- Application Supplier NoteChIP assay: 1-10 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsChIP Chromatin ImmunoPrecipitation
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5994
- Target nameRFXAP
- Target descriptionregulatory factor X associated protein
- Target synonymsregulatory factor X-associated protein; RFX DNA-binding complex 36 kDa subunit; RFX-associated protein
- HostRabbit
- IsotypeIgG
- Protein IDO00287
- Protein NameRegulatory factor X-associated protein
- Scientific DescriptionMajor histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated ankyrin-containing protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group D. Transcript variants utilizing different polyA signals have been found for this gene. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203