IHC-P analysis of human hepatocarcinoma tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
![IHC-P analysis of human prostate carcinoma tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX31128/GTX31128_1340_IHC-P_w_23060722_616.webp "IHC-P analysis of human prostate carcinoma tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:20")
![IHC-P analysis of mouse breast tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX31128/GTX31128_1341_IHC-P_w_23060722_124.webp "IHC-P analysis of mouse breast tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100")
![IP analysis of HeLa cell lysate using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. IP reaction : 2microg antibody / 750microg lysate Loading : 25 microg Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX31128/GTX31128_1450_IP_w_23060722_419.webp "IP analysis of HeLa cell lysate using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. IP reaction : 2microg antibody / 750microg lysate Loading : 25 microg Dilution : 1:1000")
![WB analysis of 25 microg of various whole cell lysates or 0.5 microg of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX31128/GTX31128_1849_WB_w_23060722_552.webp "WB analysis of 25 microg of various whole cell lysates or 0.5 microg of purified protein (full-length His-RhoA, truncated GST-RhoB or full-length GST-RhoC fusion) per well using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Dilution : 1:1000")
![ICC/IF analysis of NIH-3T3 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX31128/GTX31128_755_ICC-IF_w_23060722_566.webp "ICC/IF analysis of NIH-3T3 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature")
![ICC/IF analysis of A431 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:50 and incubated overnight](https://www.genetex.com/upload/website/prouct_img/normal/GTX31128/GTX31128_756_ICC-IF_w_23060722_782.webp "ICC/IF analysis of A431 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:50 and incubated overnight")
![ICC/IF analysis of NIH-3T3 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:50 and incubated overnight](https://www.genetex.com/upload/website/prouct_img/normal/GTX31128/GTX31128_757_ICC-IF_w_23060722_328.webp "ICC/IF analysis of NIH-3T3 cells using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:50 and incubated overnight")
IHC-P analysis of human hepatocarcinoma tissue using GTX31128 RhoA + RhoB + RhoC antibody [1A11-4G10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100
RhoA + RhoB + RhoC antibody [1A11-4G10]
GTX31128
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
Overview
- SupplierGeneTex
- Product NameRhoA + RhoB + RhoC antibody [1A11-4G10]
- Delivery Days Customer9
- Antibody SpecificityThis antibody detects an ~20 kDa protein and shows reactivity to RhoA, RhoB, and RhoC.
- Application Supplier NoteWB: 1:500 - 1:1500. ICC/IF: 1:10-1:200. IHC-P: 1:20-1:200. IP: 2 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1A11-4G10
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG1
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203