IHC-P analysis of human prostate carcinoma tissue using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200
![IHC-P analysis of mouse liver tissue using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX31144/GTX31144_1343_IHC-P_w_23060722_536.webp "IHC-P analysis of mouse liver tissue using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:100")
![IHC-P analysis of human hepatocarcinoma tissue using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX31144/GTX31144_1344_IHC-P_w_23060722_538.webp "IHC-P analysis of human hepatocarcinoma tissue using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200")
![IP analysis of HeLa cell lysates using GTX31144 RhoA + RhoC antibody [1B3-4A10]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX31144/GTX31144_1451_IP_w_23060722_646.webp "IP analysis of HeLa cell lysates using GTX31144 RhoA + RhoC antibody [1B3-4A10]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000")
![WB analysis of 25 microg of various whole cell lysates or 0.5 microg of purified protein (RhoA, RhoB, and a GST-RhoC fusion) per well using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX31144/GTX31144_1850_WB_w_23060722_684.webp "WB analysis of 25 microg of various whole cell lysates or 0.5 microg of purified protein (RhoA, RhoB, and a GST-RhoC fusion) per well using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Dilution : 1:1000")
![ICC/IF analysis of A431 cells using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX31144/GTX31144_758_ICC-IF_w_23060722_469.webp "ICC/IF analysis of A431 cells using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4oC")
![ICC/IF analysis of HeLa cells using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX31144/GTX31144_759_ICC-IF_w_23060722_700.webp "ICC/IF analysis of HeLa cells using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 and incubated overnight at 4oC")
![ICC/IF analysis of HeLa cells using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX31144/GTX31144_760_ICC-IF_w_23060722_957.webp "ICC/IF analysis of HeLa cells using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 for at least 1 hour at room temperature")
IHC-P analysis of human prostate carcinoma tissue using GTX31144 RhoA + RhoC antibody [1B3-4A10]. Right : Primary antibody Left : Negative control without primary antibody Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min Dilution : 1:200
RhoA + RhoC antibody [1B3-4A10]
GTX31144
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
Overview
- SupplierGeneTex
- Product NameRhoA + RhoC antibody [1B3-4A10]
- Delivery Days Customer9
- Antibody SpecificityThis antibody detects GTPases RhoA and RhoC, but does not react with RhoB.
- Application Supplier NoteWB: 1:500 - 1:1500. ICC/IF: 1:20-1:200. IHC-P: 1:50-1:500. IP: 2 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID1B3-4A10
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203