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Ribonuclease A / RNASE1 Antibody (Biotin)

LS-C147650
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetRNASE1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Ribonuclease A / RNASE1 Antibody (Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e. g., ELISA and Western blotting and immunofluorescence or histochemical techniques). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. ELISA, IF, IHC, WB This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e. g., ELISA and Western blotting and immunofluorescence or histochemical techniques). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID6035
  • Target name
    RNASE1
  • Target description
    ribonuclease A family member 1, pancreatic
  • Target synonyms
    HP-RNase; RAC1; RIB1; RIB-1; ribonuclease 1; ribonuclease A C1; ribonuclease pancreatic; ribonuclease, RNase A family, 1 (pancreatic); RNase 1; RNase A; RNase upI-1; RNS1
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNASE1 using anti-RNASE1 antibody (A03553-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNASE1 antigen affinity purified polyclonal antibody (Catalog # A03553-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNASE1 at approximately 18 kDa. The expected band size for RNASE1 is at 18 kDa.
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