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ProductsBack/RIPK2 Antibody - C-terminal region (OAAB17272)

RIPK2 Antibody - C-terminal region (OAAB17272)

OAAB17272
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRIPK2
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    RIPK2 Antibody - C-terminal region (OAAB17272)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Liquid. PBS with 0.09% (W/V) sodium azide.
  • Gene ID8767
  • Target name
    RIPK2
  • Target description
    receptor interacting serine/threonine kinase 2
  • Target synonyms
    CARD3; CARD-carrying kinase; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; CARDIAK; CCK; GIG30; growth-inhibiting gene 30; receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase; receptor-interacting protein 2; receptor-interacting serine/threonine-protein kinase 2; RICK; RIP2; RIP-2; tyrosine-protein kinase RIPK2
  • Host
    Rabbit
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RIP2/RIPK2 using anti-RIP2/RIPK2 antibody (A00818-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RIP2/RIPK2 antigen affinity purified polyclonal antibody (Catalog # A00818-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RIP2/RIPK2 at approximately 61 kDa. The expected band size for RIP2/RIPK2 is at 61 kDa.
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