Belgium

Luxembourg

Netherlands

DNA / RNA / Vectors

Jena Bioscience

Supplier

Research Use Only

Certification

Bio-Connect Life Sciences

Business unit

RNT-202-L | HighYield T7 P&L RNA NMR Kit (5F-UTP), Synthesis of 5-Fluoro-modified RNA | Jena Bioscience products available at Bio-Connect

HighYield T7 P&L RNA NMR Kit (5F-UTP)... | RNT-202-L | Jena Bioscience

HighYield T7 P&L RNA NMR Kit (5F-UTP), Synthesis of 5-Fluoro-modified RNA

HighYield T7 P&L RNA NMR Kit (5F-UTP) is designed to produce large amounts of 5-Fluoro-modified RNA via in vitro transcription with T7 RNA polymerase. The resulting internally modified (m)RNA can subsequently be used for structure determination via nuclear magnetic resonance (NMR) spectroscopy. 5F-UTP is incorporated instead of its natural counterpart in a non-perturbing way (intact base pairing properties). 5-Fluoro-modification simultaneously functions as a sensitive NMR reporter group (larger chemical shift dispersion than 1H). The modified T7 RNA polymerase (T7 P&L RNA polymerase) with proline 266 replaced by leucine (P266L) has been associated with increased 5 homogeneity of transcripts especially when synthesized from A-initiating phi2.5 promoter. The kit contains sufficient reagents for 15 reactions (S-Pack) or 50 reactions (L-Pack) a 20 microl (7.5 mM GTP, 7.5 mM 5-Fluoro-UTP, 7.5 mM ATP, 7.5 mM CTP). An individual optimization of 5-Fluoro-UTP concentration can easily be achieved with the single nucleotide format. A 20 microl reaction yields about 80 microg RNA after 30 min incubation (1 microg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).

RNT-202-S | HighYield T7 P&L RNA NMR Kit (5F-UTP), Synthesis of 5-Fluoro-modified RNA | Jena Bioscience products available at Bio-Connect

HighYield T7 P&L RNA NMR Kit (5F-UTP)... | RNT-202-S | Jena Bioscience

RNT-203-L | HighYield T7 P&L RNA NMR Kit (2F-ATP), Synthesis of 2-Fluoro-modified RNA | Jena Bioscience products available at Bio-Connect

HighYield T7 P&L RNA NMR Kit (2F-ATP)... | RNT-203-L | Jena Bioscience

HighYield T7 P&L RNA NMR Kit (2F-ATP), Synthesis of 2-Fluoro-modified RNA

HighYield T7 P&L RNA NMR Kit (2F-ATP) is designed to produce large amounts of 2-Fluoro-modified RNA via in vitro transcription with T7 RNA polymerase. The resulting internally modified (m)RNA can subsequently be used for structure determination via nuclear magnetic resonance (NMR) spectroscopy. 2F-ATP is incorporated instead of its natural counterpart in a non-perturbing way (intact base pairing properties). 2-Fluoro-modification simultaneously functions as a sensitive NMR reporter group (larger chemical shift dispersion than 1H). The modified T7 RNA polymerase (T7 P&L RNA polymerase) with proline 266 replaced by leucine (P266L) has been associated with increased 5 homogeneity of transcripts especially when synthesized from A-initiating phi2.5 promoter. The kit contains sufficient reagents for 15 reactions (S-Pack) or 50 reactions (L-Pack) a 20 microl (7.5 mM GTP, 7.5 mM 2-Fluoro-ATP, 7.5 mM UTP, 7.5 mM CTP). An individual optimization of 2-Fluoro-ATP concentration can easily be achieved with the single nucleotide format. A 20 microl reaction yields about 80 microg RNA after 30 min incubation (1 microg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).

RNT-203-S | HighYield T7 P&L RNA NMR Kit (2F-ATP), Synthesis of 2-Fluoro-modified RNA | Jena Bioscience products available at Bio-Connect

HighYield T7 P&L RNA NMR Kit (2F-ATP)... | RNT-203-S | Jena Bioscience

RNT-301 | HighYield T73M Aptamer Synthesis Kit (2F-dATP), Synthesis of 2-Fluoro-modified RNA | Jena Bioscience products available at Bio-Connect

HighYield T73M Aptamer Synthesis Kit (2... | RNT-301 | Jena Bioscience

HighYield T73M Aptamer Synthesis Kit (2F-dATP), Synthesis of 2-Fluoro-modified RNA

HighYield T73M Aptamer Synthesis Kit (2F-dATP) is designed to produce large amounts of 2-Fluoro-modified RNA via in vitro transcription with T73M RNA polymerase. 2-fluoro modified RNA exhibits an increased RNA stability and is ideally suited for aptamer preparation. A modified T7 RNA polymerase (T73M RNA polymerase) ensures the efficient incorporation of 2-fluoro modified dATP instead of is natural counterpart ATP. The kit contains sufficient reagents for 30 reactions a 20 microl (7.5 mM GTP, 7.5 mM 2-Fluoro-dATP, 7.5 mM UTP, 7.5 mM CTP). An individual optimization of 2-Fluoro-dATP concentration can easily be achieved with the single nucleotide format. A 20 microl reaction yields about 20 microg RNA after 30 min incubation (1 microg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).

RNT-302 | HighYield T73M Aptamer Synthesis Kit (2F-dCTP), Synthesis of 2-Fluoro-modified RNA | Jena Bioscience products available at Bio-Connect

HighYield T73M Aptamer Synthesis Kit (2... | RNT-302 | Jena Bioscience

HighYield T73M Aptamer Synthesis Kit (2F-dCTP), Synthesis of 2-Fluoro-modified RNA

HighYield T73M Aptamer Synthesis Kit (2F-dCTP) is designed to produce large amounts of 2-Fluoro-modified RNA via in vitro transcription with T73M RNA polymerase. 2-fluoro modified RNA exhibits an increased RNA stability and is ideally suited for aptamer preparation. A modified T7 RNA polymerase (T73M RNA polymerase) ensures the efficient incorporation of 2-fluoro modified dCTP instead of is natural counterpart CTP. The kit contains sufficient reagents for 30 reactions a 20 microl (7.5 mM ATP, 7.5 mM GTP, 7.5 mM 2-Fluoro-dCTP, 7.5 mM UTP). An individual optimization of 2-Fluoro-dCTP concentration can easily be achieved with the single nucleotide format. A 20 microl reaction yields about 20 microg RNA after 30 min incubation (1 microg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).

RNT-303 | HighYield T73M Aptamer Synthesis Kit (2F-dGTP), Synthesis of 2-Fluoro-modified RNA | Jena Bioscience products available at Bio-Connect

HighYield T73M Aptamer Synthesis Kit (2... | RNT-303 | Jena Bioscience

HighYield T73M Aptamer Synthesis Kit (2F-dGTP), Synthesis of 2-Fluoro-modified RNA

HighYield T73M Aptamer Synthesis Kit (2F-dGTP) is designed to produce large amounts of 2-Fluoro-modified RNA via in vitro transcription with T73M RNA polymerase. 2-fluoro modified RNA exhibits an increased RNA stability and is ideally suited for aptamer preparation. A modified T7 RNA polymerase (T73M RNA polymerase) ensures the efficient incorporation of 2-fluoro modified dGTP instead of is natural counterpart GTP. The kit contains sufficient reagents for 30 reactions a 20 microl (7.5 mM ATP, 7.5 mM CTP, 7.5 mM 2-Fluoro-dGTP, 7.5 mM UTP). An individual optimization of 2-Fluoro-dGTP concentration can easily be achieved with the single nucleotide format. A 20 microl reaction yields about 30 microg RNA after 30 min incubation (1 microg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).

RNT-304 | HighYield T73M Aptamer Synthesis Kit (2F-dUTP), Synthesis of 2-Fluoro-modified RNA | Jena Bioscience products available at Bio-Connect

HighYield T73M Aptamer Synthesis Kit (2... | RNT-304 | Jena Bioscience

HighYield T73M Aptamer Synthesis Kit (2F-dUTP), Synthesis of 2-Fluoro-modified RNA

HighYield T73M Aptamer Synthesis Kit (2F-dUTP) is designed to produce large amounts of 2-Fluoro-modified RNA via in vitro transcription with T73M RNA polymerase. 2-fluoro modified RNA exhibits an increased RNA stability and is ideally suited for aptamer preparation. A modified T7 RNA polymerase (T73M RNA polymerase) ensures the efficient incorporation of 2-fluoro modified dUTP instead of is natural counterpart UTP. The kit contains sufficient reagents for 30 reactions a 20 microl (7.5 mM ATP, 7.5 mM CTP, 7.5 mM GTP, 7.5 mM 2-Fluoro-dUTP). An individual optimization of 2-Fluoro-dUTP concentration can easily be achieved with the single nucleotide format. A 20 microl reaction yields about 20 microg RNA after 30 min incubation (1 microg T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).

Website

Tech support

Our company

Our team

Divisions

Vacatures

Events

Promotions

About us

Contact

Our people

Antibodies

Chemicals

Proteins / Signaling Molecules

Assays

(Cell) Culture

Products

Our Company

Suppliers

News

Bio-Connect

Biotech, Pharma & CRO

Academia

Diagnostics

Environment

Food

Veterinary

Industries

Disclaimer

Privacy Statement

Terms and conditions

Are you looking for specific products, alternatives, documentation or need assistance with solving product related issues? Let our in-house technical support team help you.

We support your research

<p>For 15 years, we connect life science researchers specialized in the import and distribution of life science reagents from global suppliers.</p>

Support your research

Cell Culture

Genome Editing and Engineering

Molecular Biology

Lysates / Extracts

Expression

Labware / Accesories

Body Products

Technique Specific Products

Arrays / Slides

Call +31 (0)26 326 44 50

RNA

Didn't find what you were looking for?