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PP-210L | Total RNA Purification Kit - Column Kit, Isolation of total RNA by silica-gel membrane adsorption | Jena Bioscience products available at Bio-Connect

Total RNA Purification Kit - Column Kit... | PP-210L | Jena Bioscience

Total RNA Purification Kit - Column Kit, Isolation of total RNA by silica-gel membrane adsorption

Molecular Biology

Total RNA Purification Kit is designed for rapid, high purity and high yield isolation of total RNA from small amounts of various samples including blood, animal and plant tissue, bacteria and viruses. The spin column based method allows complete removal of inhibitors such as divalent cations and proteins. Due to elimination of phenol, handling of the kit is safe and no harmful waste is produced. The purified total RNA can be used in a number of downstream applications. The kit allows the purification of up to 100 microg RNA per preparation.

PP-210S | Total RNA Purification Kit - Column Kit, Isolation of total RNA by silica-gel membrane adsorption | Jena Bioscience products available at Bio-Connect

Total RNA Purification Kit - Column Kit... | PP-210S | Jena Bioscience

PP-219 | gDNA Removal Kit, Removal of contaminating genomic DNA from RNA | Jena Bioscience products available at Bio-Connect

gDNA Removal Kit, Removal of contaminati... | PP-219 | Jena Bioscience

gDNA Removal Kit, Removal of contaminating genomic DNA from RNA

gDNA Removal Kit is designed for removal of contaminating gDNA from RNA prior to reverse transcription. The kit is based on the recombinant heat labile dsDNase, which is irreversibly inactivated at moderate temperatures. This enables an inactivation step which is gentle enough to preserve both quality and quantity of all present RNA. Reverse Transcription can be performed in the same tube, thereby minimizing pipetting steps and reducing hands-on time. The protocol is recommended for removal of genomic DNA from RNA preparations prior to reverse transcription. Complete removal of genomic DNA from RNA preps Most techniques used for RNA isolation yield RNA with significant amounts of contaminating genomic DNA (gDNA), potentially resulting in false and inaccurate mRNA quantification. This is particularly a problem in the quantification of low-copy transcripts or small samples. The gDNA Removal Kit efficiently removes gDNA from RNA preps to levels below the detection limit of RT-qPCR (Figure).

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