More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/RNF74 / RAG1 Antibody (clone 1D9)

RNF74 / RAG1 Antibody (clone 1D9)

LS-C169127
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetRAG1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RNF74 / RAG1 Antibody (clone 1D9)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1d9
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5896
  • Target name
    RAG1
  • Target description
    recombination activating 1
  • Target synonyms
    RAG-1; recombination activating gene 1; recombination activating protein 1; RING finger protein 74; RNF74; V(D)J recombination-activating protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Bioss Antibodies
RNF74 Polyclonal AntibodyBS-6941R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetRAG1
  • SizePrice
Product group Antibodies
Assay Genie
RAG1 AntibodyCAB12646
TargetRAG1
  • SizePrice
Anti-RAG1 Antibody
Product group Antibodies
Antibodies.com
Anti-RAG1 AntibodyA87856
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
RayBiotech
Anti-RAG1 Antibody144-62412
ApplicationsWestern Blot
TargetRAG1
  • SizePrice
Figure 1. Western blot analysis of RAG1 using anti-RAG1 antibody (A00307-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAG1 antigen affinity purified polyclonal antibody (Catalog # A00307-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAG1 at approximately 120KD. The expected band size for RAG1 is at 120KD.
Product group Antibodies
Boster Bio
Anti-RAG1 Antibody Picoband(r)A00307-1-CARRIER-FREE
ApplicationsWestern Blot
TargetRAG1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
RNF74 / RAG1 AntibodyLS-C747737
ApplicationsWestern Blot
TargetRAG1
  • SizePrice
Product group Antibodies
GeneTex
RAG1 antibodyGTX132124
ApplicationsWestern Blot
TargetRAG1
  • SizePrice
Product group Antibodies
Cusabio
RAG1 AntibodyCSB-PA312483XA01OFG
ApplicationsWestern Blot, ELISA
ReactivityPlant
  • SizePrice