![WB using Anti-ROBO-1 antibody shows detection of a band at ~181 kDa corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at RTfollowed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. WB using Anti-ROBO-1 antibody shows detection of a band at ~181 kDa corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at RTfollowed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.](https://cdn.origene.com/assets/images/antibody/115/600-401-692-1-w.jpg)
WB using Anti-ROBO-1 antibody shows detection of a band at ~181 kDa corresponding to ROBO-1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at RTfollowed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
ROBO1 Rabbit Polyclonal Antibody
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TA319298
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ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetROBO1
Overview
- SupplierOriGene
- Product NameROBO1 Rabbit Polyclonal Antibody
- Delivery Days Customer14
- ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Gene ID6091
- Target nameROBO1
- Target descriptionroundabout guidance receptor 1
- Target synonymsdeleted in U twenty twenty; DUTT1; roundabout homolog 1; roundabout, axon guidance receptor, homolog 1; SAX3
- HostRabbit
- IsotypeIgG
- Protein IDQ9Y6N7
- Protein NameRoundabout homolog 1
- Scientific DescriptionRabbit polyclonal anti-ROBO-1 antibody
- ReactivityCanine, Human, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203