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WB analysis of Jurkat cell lysate using GTX85485 RPA Interacting Protein antibody. Working concentration : (A) 0.5 and (B) 1 microg/ml
WB analysis of Jurkat cell lysate using GTX85485 RPA Interacting Protein antibody. Working concentration : (A) 0.5 and (B) 1 microg/ml
WB analysis of Jurkat cell lysate using GTX85485 RPA Interacting Protein antibody. Working concentration : (A) 0.5 and (B) 1 microg/ml

RPA Interacting Protein antibody

GTX85485
GeneTex
ApplicationsWestern Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetRPAIN
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Overview

  • Supplier
    GeneTex
  • Product Name
    RPA Interacting Protein antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.5 - 1 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID84268
  • Target name
    RPAIN
  • Target description
    RPA interacting protein
  • Target synonyms
    HRIP; nuclear transporter; RAP interaction protein; RIP; RPA-interacting protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86UA6
  • Protein Name
    RPA-interacting protein
  • Scientific Description
    Replication protein A (RPA) is a single-stranded-DNA binding protein involved in numerous eukaryotic DNA processes including replication, repair and recombination. RPA interacting protein (RPA IP) has been identified as an adapter protein that is involved in RPA nuclear import instead of the prototypical importin proteins that normally mediate nuclear import (2). Multiple isoforms of RPA IP are known to exist, with the longest isoform localized to the cytoplasm. Isoform 2 is sumoylated and is located in the PML nuclear body within the nucleus. It has been suggested that this isoform mediates the localization of the RPA complex into the PML nuclear body, thereby participating in RPA function in DNA metabolism.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RPAIN using anti-RPAIN antibody (A11439-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: mouse ovary tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPAIN antigen affinity purified polyclonal antibody (Catalog # A11439-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPAIN at approximately 35 kDa. The expected band size for RPAIN is at 25 kDa.
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