WB analysis of 20microg RPE65 overexpressing COS-7 cell (lane 1) and 5microg of bovine retinal pigment epithelium membrane fraction (lane 2) using GTX13826 RPE65 antibody [401.8B11.3D9].
![ICC/IF analysis of ARPE-19 cells using GTX13826 RPE65 antibody [401.8B11.3D9]. Dilution : 1:100 Fixation : 4% PFA Permibilization : 0.2% Triton X-100](https://www.genetex.com/upload/website/prouct_img/normal/GTX13826/GTX13826_128_ICC-IF_w_23060620_626.webp "ICC/IF analysis of ARPE-19 cells using GTX13826 RPE65 antibody [401.8B11.3D9]. Dilution : 1:100 Fixation : 4% PFA Permibilization : 0.2% Triton X-100")
![IHC-Fr analysis of mouse retina tissue using GTX13826 RPE65 antibody [401.8B11.3D9].](https://www.genetex.com/upload/website/prouct_img/normal/GTX13826/GTX13826_359_IHC-Fr_w_23060620_372.webp "IHC-Fr analysis of mouse retina tissue using GTX13826 RPE65 antibody [401.8B11.3D9].")
![IHC-P analysis of human glioblastoma tissue using GTX13826 RPE65 antibody [401.8B11.3D9]. Dilution : 1:500 Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)](https://www.genetex.com/upload/website/prouct_img/normal/GTX13826/GTX13826_432_IHC-P_w_23060620_382.webp "IHC-P analysis of human glioblastoma tissue using GTX13826 RPE65 antibody [401.8B11.3D9]. Dilution : 1:500 Antigen retrieval : Heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0)")
WB analysis of 20microg RPE65 overexpressing COS-7 cell (lane 1) and 5microg of bovine retinal pigment epithelium membrane fraction (lane 2) using GTX13826 RPE65 antibody [401.8B11.3D9].
RPE65 antibody [401.8B11.3D9]
GTX13826
ApplicationsFlow Cytometry, ImmunoFluorescence, Mass Spectrometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rat, Xenopus
TargetRPE65
Overview
- SupplierGeneTex
- Product NameRPE65 antibody [401.8B11.3D9] - Orthogonal Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:1000. ICC/IF: 1:10 - 1:500. IHC-P: 1:250 - 1:500. IHC-Fr: 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Mass Spectrometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID401.8B11.3D9
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID282043
- Target nameRPE65
- Target descriptionretinoid isomerohydrolase RPE65
- Target synonymsall-trans-retinyl-palmitate hydrolase; lutein isomerase; meso-zeaxanthin isomerase; retinal pigment epithelium-specific 65 kDa protein; retinal pigment epithelium-specific protein (65kD); retinal pigment epithelium-specific protein 65kDa; retinoid isomerohydrolase; retinol isomerase; RPE65, retinoid isomerohydrolase
- HostMouse
- IsotypeIgG1
- Protein IDQ28175
- Protein NameRetinoid isomerohydrolase
- Scientific DescriptionPlays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.
- ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rat, Xenopus
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- METTL3-mediated m6A modification of HMGA2 mRNA promotes subretinal fibrosis and epithelial-mesenchymal transition. Wang Y et al., 2023 Mar 21, J Mol Cell BiolRead more
- BESTROPHIN1 mutations cause defective chloride conductance in patient stem cell-derived RPE. Moshfegh Y et al., 2016 Jul 1, Hum Mol GenetRead more