Bio-Connect
Western Blot analysis of NIH/3T3, A431 and Jurkat cell using RPLP0 Polyclonal Antibody at dilution of 1:750
Western Blot analysis of NIH/3T3, A431 and Jurkat cell using RPLP0 Polyclonal Antibody at dilution of 1:750
Western Blot analysis of NIH/3T3, A431 and Jurkat cell using RPLP0 Polyclonal Antibody at dilution of 1:750

RPLP0 Polyclonal Antibody

E-AB-10583
Elabscience
Product group Antibodies
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Elabscience
  • Product Name
    RPLP0 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
  • UNSPSC
    12352203

Related products

Western Blot Positive WB detected in: Rat brain tissue, Rat kidney tissue, Mouse kidney tissue All lanes: RPLP0 antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 28 kDa Observed band size: 35 kDa
Product group Antibodies
Cusabio
RPLP0 AntibodyCSB-PA020336LA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetRPLP0
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetRPLP0
  • SizePrice
Product group Antibodies
Assay Genie
TargetRPLP0
  • SizePrice
Product group Antibodies
References
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetRPLP0
  • SizePrice
Immunohistochemical staining of human breast shows moderate cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
TargetRPLP0
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetRPLP0
  • SizePrice
Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-RPLP0 Polyclonal Antibody, Unconjugated (bs-1464R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetRPLP0
  • SizePrice
Figure 1. Western blot analysis of RPLP0 using anti-RPLP0 antibody (A04349-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A375 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPLP0 antigen affinity purified polyclonal antibody (Catalog # A04349-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPLP0 at approximately 37 kDa. The expected band size for RPLP0 is at 34 kDa.
Product group Antibodies
Boster Bio
Anti-RPLP0 Antibody Picoband(r)A04349-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetRPLP0
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
RPLP0 AntibodyLS-C401015
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetRPLP0
  • SizePrice