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RRP1 Antibody (Internal)

LS-C155262
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetRRP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RRP1 Antibody (Internal)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suggested starting dilutions are as follows: Western blot: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:3000) Suggested starting dilutions are as follows: Western blot: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.64 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8568
  • Target name
    RRP1
  • Target description
    ribosomal RNA processing 1
  • Target synonyms
    D21S2056E; NNP-1; Nnp1 homolog, nucleolar protein; NOP52; novel nuclear protein 1; nucleolar protein Nop52; protein NNP-1; ribosomal RNA processing 1 homolog; ribosomal RNA processing protein 1 homolog A; RRP1A; RRP1-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RRP1 using anti-RRP1 antibody (A06230-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RRP1 antigen affinity purified polyclonal antibody (Catalog # A06230-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RRP1 at approximately 53 kDa. The expected band size for RRP1 is at 53 kDa.
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