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ProductsBack/RTFDC1 Antibody (clone OTI7E10)

RTFDC1 Antibody (clone OTI7E10)

LS-C172676
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetRTF2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RTFDC1 Antibody (clone OTI7E10)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:100), WB (1:1000 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI7E10
  • Concentration
    0.59 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51507
  • Target name
    RTF2
  • Target description
    replication termination factor 2
  • Target synonyms
    C20orf43; CDAO5; HSPC164; protein RTF2 homolog; replication termination factor 2; replication termination factor 2 domain containing 1; replication termination factor 2 domain-containing protein 1; RTFDC1; SHUJUN-3; UPF0549 protein C20orf43
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RTF2 using anti-RTF2 antibody (A16683-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Siha whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTF2 antigen affinity purified polyclonal antibody (Catalog # A16683-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTF2 at approximately 37 kDa. The expected band size for RTF2 is at 34 kDa.
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