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ProductsBack/RTN4R Antibody (Biotin)

RTN4R Antibody (Biotin)

LS-C183981
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetRTN4R
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    RTN4R Antibody (Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot
  • Applications Supplier
    WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID65078
  • Target name
    RTN4R
  • Target description
    reticulon 4 receptor
  • Target synonyms
    NGR; nogo receptor; Nogo-66 receptor; NOGOR; reticulon-4 receptor; UNQ330/PRO526
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA580390(RTN4R Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
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Figure 1. Western blot analysis of Nogo Receptor/NgR1/RTN4R using anti-Nogo Receptor/NgR1/RTN4R antibody (A02250-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nogo Receptor/NgR1/RTN4R antigen affinity purified polyclonal antibody (Catalog # A02250-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nogo Receptor/NgR1/RTN4R at approximately 70 kDa. The expected band size for Nogo Receptor/NgR1/RTN4R is at 63 kDa.
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