rTth DNA Polymerase

Cosmo Bio USA

rTth DNA Polymerase

16

Research Use Only

For PCR,RT-PCR, dye-deoxy sequencing, creation of double stranded DNA by origonucleotide as primers Tth DNA polymerase is a thermostable DNA polymerase derived from the thermophilic bacteria Thermus thermophilus (Tth) HB8. The enzyme has a reverse transcriptase activity in addition to a 5- 3 polymerase activity and a double strand specific 5- 3 exonuclease activity in the presence of Mn2+ ions. Therefore, this enzyme enables one-step RT-PCR including the reverse transcription and PCR steps. Kits for one-step RT-PCR (Code No. PCR-311) and real-time PCR (Code No. QRT-101, 201) using this enzyme are also available. Features: Exhibits reverse transcriptase activity in the presence of Mn2+ ions. Effective for the amplification of GC-rich targets and crude samples. Effective for reverse transcription of RNA with complicated secondary structure due to the reaction occurring at high temperature (i.e., 60oC). Application: PCR RT-PCR Primer extension

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