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Immunofluorescence analysis of HuvEc cells, using AOS1 antibody.
Immunofluorescence analysis of HuvEc cells, using AOS1 antibody.
Immunofluorescence analysis of HuvEc cells, using AOS1 antibody.

SAE1 Antibody

CSB-PA794022
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSAE1
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Overview

  • Supplier
    Cusabio
  • Product Name
    SAE1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10055
  • Target name
    SAE1
  • Target description
    SUMO1 activating enzyme subunit 1
  • Target synonyms
    activator of SUMO1; AOS1; HSPC140; sentrin/SUMO-activating protein AOS1; SUA1; SUMO-1 activating enzyme E1 N subunit; SUMO-1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme; UBLE1A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBE0
  • Protein Name
    SUMO-activating enzyme subunit 1
  • Scientific Description
    The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. Yoshiaki Azuma, FASEB J, Jun 2001; 10.1096. Walter Makous, Invest. Ophthalmol. Vis. Sci., Sep 2006; 47: 4160 - 4167. Hsin-Pei Shih, J. Cell Sci., Mar 2002; 115: 1259. Thomas Buschmann, J. Biol. Chem., Oct 2001; 276: 40389 - 40395.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SAE1 using anti-SAE1 antibody (A04753-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAE1 antigen affinity purified polyclonal antibody (Catalog # A04753-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAE1 at approximately 38 kDa. The expected band size for SAE1 is at 38 kDa.
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