GTX65630-pro Salmonella typhimurium Flagellin protein activates TLR5 in vivo. Mice WT are injected i.v. with indicated doses of Flagellin protein. After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10microg of Flagellin protein, but no expression of CCL20 and IL-6 could be observed (not shown).
GTX65630-pro Salmonella typhimurium Flagellin protein activates TLR5 in vivo. Mice WT are injected i.v. with indicated doses of Flagellin protein. After 2 hours, levels of CCL20 and IL-6 in the serum were measured by ELISA. As a control, mice TLR5 -/- have been injected i.v. with 10microg of Flagellin protein, but no expression of CCL20 and IL-6 could be observed (not shown).
Salmonella typhimurium Flagellin protein (active)
GTX65630-PRO
Overview
- SupplierGeneTex
- Product NameSalmonella typhimurium Flagellin protein (active)
- Delivery Days Customer9
- Application Supplier NoteActivation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and ma- trilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1microg /mouse.
- ApplicationsFunctional Assay
- CertificationResearch Use Only
- ConjugateUnconjugated
- FormulationLyophilized
- Storage Instruction-20°C
- UNSPSC12352202