Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SART3 antibody [C3], C-term (GTX107684) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![SART3 antibody [C3], C-term immunoprecipitates SART3 protein in IP experiments. IP samples: HepG2 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of SART3 protein by 4 μg SART3 antibody [C3], C-term (GTX107684) 5 % SDS-PAGE The immunoprecipitated SART3 protein was detected by SART3 antibody [C3], C-term (GTX107684) diluted at 1:2000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX107684/GTX107684_39757_IP_w_23060120_562.webp "SART3 antibody [C3], C-term immunoprecipitates SART3 protein in IP experiments. IP samples: HepG2 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of SART3 protein by 4 μg SART3 antibody [C3], C-term (GTX107684) 5 % SDS-PAGE The immunoprecipitated SART3 protein was detected by SART3 antibody [C3], C-term (GTX107684) diluted at 1:2000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SART3 antibody [C3], C-term (GTX107684) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX107684/GTX107684_39757_20241115_WB_TPM_watermark_24111918_796.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SART3 antibody [C3], C-term (GTX107684) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SART3 antibody [C3], C-term (GTX107684) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SART3 antibody [C3], C-term
GTX107684
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSART3
Overview
- SupplierGeneTex
- Product NameSART3 antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.52 mg/ml
- ConjugateUnconjugated
- Gene ID9733
- Target nameSART3
- Target descriptionspliceosome associated factor 3, U4/U6 recycling protein
- Target synonymsDSAP1, P100, RP11-13G14, TIP110, p110, p110(nrb), spliceosome associated factor 3, U4/U6 recycling protein, HIV-1 Tat-interacting protein of 110kDa, PRP24 homolog, SART-3, hSART-3, p110 nuclear RNA-binding protein, squamous cell carcinoma antigen recognized by T cells 3, tat-interacting protein of 110 kDa
- HostRabbit
- IsotypeIgG
- Protein IDQ15020
- Protein NameSpliceosome associated factor 3, U4/U6 recycling protein
- Scientific DescriptionThe protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- El Fatimy R, Zhang Y, Deforzh E, et al. A nuclear function for an oncogenic microRNA as a modulator of snRNA and splicing. Mol Cancer. 2022,21(1):17. doi: 10.1186/s12943-022-01494-zRead this paper
- Sundararaman B, Zhan L, Blue SM, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016,61(6):903-13. doi: 10.1016/j.molcel.2016.02.012Read this paper
Datasheet
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