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Western blot analysis of extracts from normal (control) and [KO Validated] SAV1 knockout (KO) HeLa cells, using [KO Validated] SAV1 antibody (TA381263) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 90s.
Western blot analysis of extracts from normal (control) and [KO Validated] SAV1 knockout (KO) HeLa cells, using [KO Validated] SAV1 antibody (TA381263) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 90s.
Western blot analysis of extracts from normal (control) and [KO Validated] SAV1 knockout (KO) HeLa cells, using [KO Validated] SAV1 antibody (TA381263) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 90s.

SAV1 Rabbit Polyclonal Antibody

TA381263
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSAV1
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    SAV1 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID60485
  • Target name
    SAV1
  • Target description
    salvador family WW domain containing protein 1
  • Target synonyms
    SAV, WW45, WWP4, protein salvador homolog 1, 1700040G09Rik, 45 kDa WW domain protein, WW domain-containing adaptor 45, hWW45, salvador homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H4B6
  • Protein Name
    Protein salvador homolog 1
  • Scientific Description
    SAV1 Rabbit polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Western Blot Positive WB detected in: 293T whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate All lanes: SAV1 antibody at 3.64microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa
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Figure 1. Western blot analysis of SAV1 using anti-SAV1 antibody (A04183-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human DLD-1 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAV1 antigen affinity purified polyclonal antibody (Catalog # A04183-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAV1 at approximately 45 kDa. The expected band size for SAV1 is at 45 kDa.
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