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ProductsBack/SAV1 Rabbit Polyclonal Antibody
Western blot analysis of lysates from wild type (WT) and SAV1 knockout (KO) HeLa cells
Western blot analysis of lysates from wild type (WT) and SAV1 knockout (KO) HeLa cells
Western blot analysis of lysates from wild type (WT) and SAV1 knockout (KO) HeLa cells

SAV1 Rabbit Polyclonal Antibody

TA381263
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSAV1
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    SAV1 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID60485
  • Target name
    SAV1
  • Target description
    salvador family WW domain containing protein 1
  • Target synonyms
    SAV, WW45, WWP4, protein salvador homolog 1, 1700040G09Rik, 45 kDa WW domain protein, WW domain-containing adaptor 45, hWW45, salvador homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H4B6
  • Protein Name
    Protein salvador homolog 1
  • Scientific Description
    SAV1 Rabbit polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SAV1 using anti-SAV1 antibody (A04183-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human DLD-1 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human PANC-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAV1 antigen affinity purified polyclonal antibody (Catalog # A04183-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAV1 at approximately 45 kDa. The expected band size for SAV1 is at 45 kDa.
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ReactivityHuman
TargetSAV1
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Anti-SAV1 Antibody144-09980
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TargetSAV1
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Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.
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Western Blot Positive WB detected in: 293T whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate All lanes: SAV1 antibody at 3.64microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa
Product group Antibodies
Cusabio
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WB analysis of various sample lysates using GTX66596 Salvador homolog 1 antibody. Dilution : 1:1000 Loading : 25μg per lane
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Salvador homolog 1 antibodyGTX66596
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TargetSAV1
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