SCRIPT Direct RT-qPCR ProbesMaster UNG, Robust real-time RT-PCR master mix with UNG, for highly sensitive and specific amplification directly from tissue, swabs or blood

Jena Bioscience

SCRIPT Direct RT-qPCR ProbesMaster UNG, Robust real-time RT-PCR master mix with UNG, for highly sensitive and specific amplification directly from tissue, swabs or blood

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Research Use Only

SCRIPT Direct RT-qPCR ProbesMaster UNG is designed for quantitative real-time analysis of target RNA directly from animal- or plant tissue, swabs and blood. The mix allows robust amplification avoiding the requirement of any prior RNA purification procedures. The mix is recommended for use with dual-labeled fluorescent probes, e.g. TaqMan®, Molecular Beacon or Scorpion probes. It provides a powerful tool for multiplex-quantification of sample RNA in a broad dynamic range with exceptional sensitivity and precision. The mix contains all reagents required for RT-qPCR (except template, primer and labeled fluorescent probe) in a premixed 2 x concentrated ready-to-use solution. High robustness, reliability and sensitivity of the mix are based on a genetically engineered reverse transcriptase and an antibody-blocked hot start polymerase in combination with an optimized and well-balanced buffer system. The mix ensures fast and easy preparation with a minimum of pipetting steps and is specially recommended for: Direct amplification of target RNA from various tissues samplesDirect detection of viral or bacterial RNA in nasal or throat swabsDirect PCR from blood samplesPoint-of-Care diagnostics. Multiplexing Capability Real-time RT-PCR technology based on dual-labeled DNA probes provides a high sensitive and specific RT-PCR system with multiplexing capability. The simultaneous detection of multiple targets in a single tube requires a primer/probe set for each target amplification. Sequences and concentrations of primers and probes should be optimized to avoid mutual influence, secondary structures and primer-dimer formations. Amplification of each target is detected in a separate fluorescence channel. UNG (Uracil-N-Glycosylase) The mix contains UNG (Uracil-N-Glycosylase) and dUTP instead of dTTP to eliminate carry-over contamination of DNA from previous PCR reactions. The UNG treatment at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template. ROX Reference Dye The mix can also be used in combination with ROX reference dye (#PCR-351) in PCR instruments that are compatible with the evaluation of the ROX signal.

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