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Search results: AMPK alpha 1

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Figure 1. Western blot analysis of AMPK alpha 1/PRKAA1 using anti-AMPK alpha 1/PRKAA1 antibody (A00994-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: monkey Cos-7 whole cell lysates, Lane 5: human K572 whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat heart tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse heart tissue lysates, Lane 11: mouse intestines tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1/PRKAA1 antigen affinity purified polyclonal antibody (Catalog # A00994-6) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK alpha 1/PRKAA1 at approximately 64-66KD. The expected band size for AMPK alpha 1/PRKAA1 is at 64KD.
Product group Antibodies
Boster Bio
Anti-AMPK alpha 1/PRKAA1 Antibody Picoband(r)A00994-6-DYLIGHT594
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetPRKAA1
  • SizePrice
Figure 1. Western blot analysis of AMPK alpha 1/PRKAA1 using anti-AMPK alpha 1/PRKAA1 antibody (A00994-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: monkey Cos-7 whole cell lysates, Lane 5: human K572 whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat heart tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse heart tissue lysates, Lane 11: mouse intestines tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1/PRKAA1 antigen affinity purified polyclonal antibody (Catalog # A00994-6) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK alpha 1/PRKAA1 at approximately 64-66KD. The expected band size for AMPK alpha 1/PRKAA1 is at 64KD.
Product group Antibodies
Boster Bio
Anti-AMPK alpha 1/PRKAA1 Antibody Picoband(r)A00994-6-FITC
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetPRKAA1
  • SizePrice
Figure 1. Western blot analysis of AMPK alpha 1/PRKAA1 using anti-AMPK alpha 1/PRKAA1 antibody (A00994-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: monkey Cos-7 whole cell lysates, Lane 5: human K572 whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat heart tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse heart tissue lysates, Lane 11: mouse intestines tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1/PRKAA1 antigen affinity purified polyclonal antibody (Catalog # A00994-6) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK alpha 1/PRKAA1 at approximately 64-66KD. The expected band size for AMPK alpha 1/PRKAA1 is at 64KD.
Product group Antibodies
Boster Bio
Anti-AMPK alpha 1/PRKAA1 Antibody Picoband(r)A00994-6-HRP
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetPRKAA1
  • SizePrice
Figure 1. Western blot analysis of AMPK alpha 1/PRKAA1 using anti-AMPK alpha 1/PRKAA1 antibody (A00994-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: monkey Cos-7 whole cell lysates, Lane 5: human K572 whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat heart tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse heart tissue lysates, Lane 11: mouse intestines tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1/PRKAA1 antigen affinity purified polyclonal antibody (Catalog # A00994-6) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK alpha 1/PRKAA1 at approximately 64-66KD. The expected band size for AMPK alpha 1/PRKAA1 is at 64KD.
Product group Antibodies
Boster Bio
Anti-AMPK alpha 1/PRKAA1 Antibody Picoband(r)A00994-6-IFLUOR647
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetPRKAA1
  • SizePrice
Figure 1. Western blot analysis of AMPK alpha 1/PRKAA1 using anti-AMPK alpha 1/PRKAA1 antibody (A00994-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: monkey Cos-7 whole cell lysates, Lane 5: human K572 whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat heart tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse heart tissue lysates, Lane 11: mouse intestines tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1/PRKAA1 antigen affinity purified polyclonal antibody (Catalog # A00994-6) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK alpha 1/PRKAA1 at approximately 64-66KD. The expected band size for AMPK alpha 1/PRKAA1 is at 64KD.
Product group Antibodies
Boster Bio
Anti-AMPK alpha 1/PRKAA1 Antibody Picoband(r)A00994-6-PE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetPRKAA1
  • SizePrice
Figure 1. Western blot analysis of AMPK alpha 1/PRKAA1 using anti-AMPK alpha 1/PRKAA1 antibody (A00994-6). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: monkey Cos-7 whole cell lysates, Lane 5: human K572 whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat heart tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse heart tissue lysates, Lane 11: mouse intestines tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPK alpha 1/PRKAA1 antigen affinity purified polyclonal antibody (Catalog # A00994-6) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPK alpha 1/PRKAA1 at approximately 64-66KD. The expected band size for AMPK alpha 1/PRKAA1 is at 64KD.
Product group Antibodies
References
Boster Bio
Anti-AMPK alpha 1/PRKAA1 Antibody Picoband(r)A00994-6
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetPRKAA1
  • SizePrice
Product group Antibodies
Bioss
AMPK alpha-1 Polyclonal Antibody, APC-Cy7 ConjugatedBS-41337R-APC-CY7
ApplicationsWestern Blot
TargetPRKAA1
  • SizePrice
Product group Antibodies
Bioss
AMPK alpha-1 Polyclonal Antibody, PE-Cy5 ConjugatedBS-41337R-PE-CY5
ApplicationsWestern Blot
TargetPRKAA1
  • SizePrice
Product group Antibodies
Bioss
AMPK alpha-1 Polyclonal Antibody, PE-Cy7 ConjugatedBS-41337R-PE-CY7
ApplicationsWestern Blot
TargetPRKAA1
  • SizePrice
Product group Antibodies
Bioss
AMPK alpha 2 Polyclonal Antibody, APC-Cy7 ConjugatedBS-2771R-APC-CY7
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetPRKAA2
  • SizePrice
Product group Antibodies
Bioss
AMPK alpha 2 Polyclonal Antibody, PE-Cy5 Conjugatedbs-2771R-PE-Cy5
ApplicationsFlow Cytometry, Western Blot
TargetPRKAA2
  • SizePrice
Product group Antibodies
Bioss
AMPK alpha 2 Polyclonal Antibody, PE-Cy7 Conjugatedbs-2771R-PE-Cy7
ApplicationsFlow Cytometry, Western Blot
TargetPRKAA2
  • SizePrice
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