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Search results: CD134

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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
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Product group Antibodies
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ELISA
ReactivityHuman, Mouse, Rat
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Western blot analysis of TNFRSF4 expression in Human fetal spleen lysate using anti-TNFRSF4 antibody (M02495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFRSF4 antigen affinity purified polyclonal antibody (Catalog # M02495) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for TNFRSF4.
Product group Antibodies
Boster Bio
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetTNFRSF4
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Product group Antibodies
LifeSpan BioSciences
ApplicationsFlow Cytometry, ELISA
ReactivityHuman
TargetTNFRSF4
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Product group Antibodies
ABclonal
TargetTNFRSF4
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Product group Antibodies
ABclonal
TargetTNFRSF4
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Product group Proteins / Signaling Molecules
Chimerigen Laboratories
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GTX76450 FACS Image
Product group Antibodies
GeneTex
ApplicationsFlow Cytometry
ReactivityRat
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Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody (A02495). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody (Catalog # A02495) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.
Product group Antibodies
Boster Bio
Product group Assays
BioVendor
Product group Antibodies
MyBioSource
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