Bio-Connect

Search results: Drosha

13 to 24 of 393 products
Sort by
Lane 1: Hela Cells; Lane 2: SiHa Cells; Probed with Drosha (5C9) Monoclonal Antibody (bsm-54184R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Product group Antibodies
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetDROSHA
  • SizePrice
Figure 1. Western blotting validation for Anti-Ribonuclease 3 Drosha Antibody A00111-1 Western blot (WB) analysis of Drosha polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:sp2/0 cell lysate Lane3:PC12 cell lysate Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetDROSHA
  • SizePrice
Figure 2. Immunocytochemistry staining of DROSHA using Anti-Ribonuclease 3 Drosha Antibody (A00111-2). ICC staining Drosha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetDROSHA
  • SizePrice
Figure 1. Western blot analysis of DROSHA using anti-DROSHA antibody (A00111-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DROSHA antigen affinity purified polyclonal antibody (Catalog # A00111-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DROSHA at approximately 200KD. The expected band size for DROSHA is at 159KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse
TargetDROSHA
  • SizePrice
Figure 1. Western blot analysis of DROSHA using anti-DROSHA antibody (A00111-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DROSHA antigen affinity purified polyclonal antibody (Catalog # A00111-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DROSHA at approximately 200KD. The expected band size for DROSHA is at 159KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse
TargetDROSHA
  • SizePrice
Figure 1. Western blot analysis of DROSHA using anti-DROSHA antibody (A00111-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DROSHA antigen affinity purified polyclonal antibody (Catalog # A00111-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DROSHA at approximately 200KD. The expected band size for DROSHA is at 159KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse
TargetDROSHA
  • SizePrice
Figure 1. Western blot analysis of DROSHA using anti-DROSHA antibody (A00111-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DROSHA antigen affinity purified polyclonal antibody (Catalog # A00111-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DROSHA at approximately 200KD. The expected band size for DROSHA is at 159KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse
TargetDROSHA
  • SizePrice
Figure 1. Western blot analysis of DROSHA using anti-DROSHA antibody (A00111-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DROSHA antigen affinity purified polyclonal antibody (Catalog # A00111-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DROSHA at approximately 200KD. The expected band size for DROSHA is at 159KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse
TargetDROSHA
  • SizePrice
Figure 1. Western blot analysis of DROSHA using anti-DROSHA antibody (A00111-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DROSHA antigen affinity purified polyclonal antibody (Catalog # A00111-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DROSHA at approximately 200KD. The expected band size for DROSHA is at 159KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse
TargetDROSHA
  • SizePrice
Figure 1. Western blot analysis of DROSHA using anti-DROSHA antibody (A00111-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DROSHA antigen affinity purified polyclonal antibody (Catalog # A00111-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DROSHA at approximately 200KD. The expected band size for DROSHA is at 159KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse
TargetDROSHA
  • SizePrice
Figure 1. Western blot analysis of DROSHA using anti-DROSHA antibody (A00111-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DROSHA antigen affinity purified polyclonal antibody (Catalog # A00111-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DROSHA at approximately 200KD. The expected band size for DROSHA is at 159KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse
TargetDROSHA
  • SizePrice
Figure 1. Western blot analysis of DROSHA using anti-DROSHA antibody (A00111-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human SW620 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: mouse Neuro-2a whole cell lysates, Lane 8: mouse Ana-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DROSHA antigen affinity purified polyclonal antibody (Catalog # A00111-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DROSHA at approximately 200KD. The expected band size for DROSHA is at 159KD.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman, Mouse
TargetDROSHA
  • SizePrice