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Search results: FGF22

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Product group Proteins / Signaling Molecules
Biomatik
ApplicationsOther Application
Protein IDQ9HCT0
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
TargetFGF22
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
FGF22 AntibodyLS-C663278
ApplicationsWestern Blot
TargetFGF22
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
FGF22 AntibodyLS-C674034
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetFGF22
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Product group Antibodies
LifeSpan BioSciences
FGF22 AntibodyLS-C823543
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetFGF22
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
FGF22 AntibodyLS-C801745
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetFGF22
  • SizePrice
Figure 1. Western blot analysis of FGF22 using anti-FGF22 antibody (PA1455). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF22 antigen affinity purified polyclonal antibody (Catalog # PA1455) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF22 at approximately 24KD. The expected band size for FGF22 is at 20KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetFGF22
  • SizePrice
Figure 1. Western blot analysis of FGF22 using anti-FGF22 antibody (PA1455). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF22 antigen affinity purified polyclonal antibody (Catalog # PA1455) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF22 at approximately 24KD. The expected band size for FGF22 is at 20KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetFGF22
  • SizePrice
Figure 1. Western blot analysis of FGF22 using anti-FGF22 antibody (PA1455). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF22 antigen affinity purified polyclonal antibody (Catalog # PA1455) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF22 at approximately 24KD. The expected band size for FGF22 is at 20KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetFGF22
  • SizePrice
Figure 1. Western blot analysis of FGF22 using anti-FGF22 antibody (PA1455). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF22 antigen affinity purified polyclonal antibody (Catalog # PA1455) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF22 at approximately 24KD. The expected band size for FGF22 is at 20KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetFGF22
  • SizePrice
Figure 1. Western blot analysis of FGF22 using anti-FGF22 antibody (PA1455). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF22 antigen affinity purified polyclonal antibody (Catalog # PA1455) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF22 at approximately 24KD. The expected band size for FGF22 is at 20KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetFGF22
  • SizePrice
Figure 1. Western blot analysis of FGF22 using anti-FGF22 antibody (PA1455). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF22 antigen affinity purified polyclonal antibody (Catalog # PA1455) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF22 at approximately 24KD. The expected band size for FGF22 is at 20KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetFGF22
  • SizePrice

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