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Search results: FRS2

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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
ReactivityMouse
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
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Western blot analysis of FRS2 expression in HeLa cell lysate using anti-FRS2 antibody (M02798). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRS2 antigen affinity purified polyclonal antibody (Catalog # M02798) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for FRS2.
Product group Antibodies
Boster Bio
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with FRS2(Tyr436) Polyclonal Antibody (bs-7902R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
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Product group Assays
LifeSpan BioSciences
ReactivityMouse
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Product group Assays
LifeSpan BioSciences
ReactivityMouse
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Product group Assays
LifeSpan BioSciences
ReactivityHuman
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Product group Assays
LifeSpan BioSciences
ReactivityHuman
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