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Search results: GAA

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Immunohistochemical staining of human epididymis shows strong granular cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-GAAHPA026970
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetGAA
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Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetGAA
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Product group Antibodies
Biomatik
GAA antibodyCAF50543
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetGAA
  • SizePrice
Western blot analysis of GAA using anti-GAA antibody (A01548). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAA antigen affinity purified polyclonal antibody (Catalog # A01548) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAA at approximately 110,95,76,70KD. The expected band size for GAA is at 110,95,76,70KD.
Product group Antibodies
Boster Bio
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetGAA
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Product group Antibodies
Reddot Biotech
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetGAA
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Rat
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Product group Assays
Wuhan Fine Biotech
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Product group Assays
Wuhan Fine Biotech
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Product group Antibodies
LifeSpan BioSciences
ApplicationsELISA
ReactivityHuman
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Product group Antibodies
LifeSpan BioSciences
ApplicationsELISA
ReactivityHuman
  • SizePrice