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Search results: GAA

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Figure 1. Western blot analysis of GAA using anti-GAA antibody (A01548). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAA antigen affinity purified polyclonal antibody (Catalog # A01548) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAA at approximately 110,95,76,70KD. The expected band size for GAA is at 110,95,76,70KD.
Product group Antibodies
Boster Bio
Anti-GAA Antibody Picoband(r)A01548-PE
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetGAA
  • SizePrice
Figure 1. Western blot analysis of GAA using anti-GAA antibody (A01548). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAA antigen affinity purified polyclonal antibody (Catalog # A01548) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAA at approximately 110,95,76,70KD. The expected band size for GAA is at 110,95,76,70KD.
Product group Antibodies
References
Boster Bio
Anti-GAA Antibody Picoband(r)A01548
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetGAA
  • SizePrice
Product group Chemicals
Biosynth
Coulteropine [6014-62-6]GAA01462
CAS Number6014-62-6
Molecular Weight383.40 g/mol
  • SizePrice
Product group Chemicals
Biosynth
Butanilicaine hydrochloride [6027-28-7]GAA02728
CAS Number6027-28-7
Molecular Weight291.21 g/mol
  • SizePrice
Product group Chemicals
Biosynth
Cucurbitacin A [6040-19-3]GAA04019
CAS Number6040-19-3
Molecular Weight574.71 g/mol
  • SizePrice
Product group Chemicals
Biosynth
Phenacyltriphenylphosphonium Bromide [6048-29-9]GAA04829
CAS Number6048-29-9
Molecular Weight461.34 g/mol
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Mouse, GAA cDNA ORF Clone, C-His tagMG57827-CH
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Mouse, GAA cDNA ORF Clone, C-Myc tagMG57827-CM
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Mouse, GAA cDNA ORF Clone, C-HA tagMG57827-CY
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Mouse, GAA cDNA ORF Clone, N-His tagMG57827-NH
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Mouse, GAA cDNA ORF Clone, N-Myc tagMG57827-NM
CategoryDNA / RNA / Vectors
  • SizePrice
Product group DNA / RNA / Vectors
Sino Biological
Mouse, GAA cDNA ORF Clone, N-HA tagMG57827-NY
CategoryDNA / RNA / Vectors
  • SizePrice
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