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Search results: GATA4

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Figure 1. Western blot analysis of GATA4 using anti-GATA4 antibody (A00499-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA4 antigen affinity purified polyclonal antibody (Catalog # A00499-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA4 at approximately 45KD. The expected band size for GATA4 is at 45KD.
Product group Antibodies
Boster Bio
Anti-GATA4 Antibody Picoband(r)A00499-1-HRP
ApplicationsWestern Blot
TargetGATA4
  • SizePrice
Figure 1. Western blot analysis of GATA4 using anti-GATA4 antibody (A00499-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA4 antigen affinity purified polyclonal antibody (Catalog # A00499-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA4 at approximately 45KD. The expected band size for GATA4 is at 45KD.
Product group Antibodies
Boster Bio
Anti-GATA4 Antibody Picoband(r)A00499-1-IFLUOR647
ApplicationsWestern Blot
TargetGATA4
  • SizePrice
Figure 1. Western blot analysis of GATA4 using anti-GATA4 antibody (A00499-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GATA4 antigen affinity purified polyclonal antibody (Catalog # A00499-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GATA4 at approximately 45KD. The expected band size for GATA4 is at 45KD.
Product group Antibodies
Boster Bio
Anti-GATA4 Antibody Picoband(r)A00499-1-PE
ApplicationsWestern Blot
TargetGATA4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
GATA4 AntibodyLS-C812619
ApplicationsWestern Blot, ELISA
TargetGATA4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
GATA4 AntibodyLS-C815319
ApplicationsImmunoPrecipitation, Western Blot
TargetGATA4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
GATA4 AntibodyLS-C822737
ApplicationsWestern Blot, ELISA
TargetGATA4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
GATA4 AntibodyLS-C833164
ApplicationsWestern Blot
TargetGATA4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
GATA4 AntibodyLS-C748787
ApplicationsWestern Blot
TargetGATA4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
GATA4 AntibodyLS-C754884
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetGATA4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
GATA4 AntibodyLS-C801358
ApplicationsImmunoFluorescence, Western Blot, ELISA
TargetGATA4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
GATA4 AntibodyLS-C417065
ApplicationsWestern Blot
TargetGATA4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
GATA4 AntibodyLS-C417536
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetGATA4
  • SizePrice
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