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Search results: H2AFX

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Product group Antibodies
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetH2AX
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Product group Antibodies
Aviva Systems Biology
ApplicationsWestern Blot
TargetH2ax
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Product group Antibodies
Biorbyt
ApplicationsWestern Blot
TargetH2AX
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Product group Proteins / Signaling Molecules
LifeSpan BioSciences
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Figure 1. Western blot analysis of H2AFX using anti-H2AFX antibody (M00241-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse thymus tissues lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H2AFX antigen affinity purified monoclonal antibody (Catalog # M00241-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H2AFX at approximately 15 kDa. The expected band size for H2AFX is at 15 kDa.
Product group Antibodies
Boster Bio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetH2AX
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Product group Proteins / Signaling Molecules
Biorbyt
Protein IDP16104
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Figure 1. ICC result Immunocytochemistry of HeLa cells, using Anti-Phospho-Histone H2A/H4 (Ser1) Rabbit mAb RM216 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, Other Application
TargetH2AC1
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Product group Proteins / Signaling Molecules
Aviva Systems Biology
ApplicationsWestern Blot
Protein IDP16104
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Product group Assays
LifeSpan BioSciences
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Product group Antibodies
MyBioSource
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Product group Antibodies
MyBioSource
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Product group Antibodies
MyBioSource
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