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Search results: H2AFX

253 to 264 of 1.001 products
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Product group Antibodies
Biorbyt
H2AFX antibodyORB167529
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetH2AX
  • SizePrice
Product group Antibodies
Biorbyt
H2AFX antibodyORB539764
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetH2AX
  • SizePrice
Product group Antibodies
Biorbyt
H2AFX AntibodyORB513195
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Proteins / Signaling Molecules
Biorbyt
H2AFX peptideORB217387
ApplicationsNeutralisation/Blocking
Protein IDP16104
  • SizePrice
Product group Antibodies
Biorbyt
H2AFX antibodyORB31803
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetH2AX
  • SizePrice
Figure 1. Western blot analysis of H2AFX using anti-H2AFX antibody (M00241-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse thymus tissues lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-H2AFX antigen affinity purified monoclonal antibody (Catalog # M00241-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for H2AFX at approximately 15 kDa. The expected band size for H2AFX is at 15 kDa.
Product group Antibodies
Boster Bio
Anti-Histone H2A.X H2AFX Rabbit Monoclonal AntibodyM00241-1
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetH2AX
  • SizePrice
Product group Proteins / Signaling Molecules
Aviva Systems Biology
H2AFX Peptide - middle region (AAP58281)AAP58281
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Protein IDP16104
  • SizePrice
Product group Antibodies
Biorbyt
H2AFX Antibody - middle region (ARP58281_P050)ORB583941
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetH2AX
  • SizePrice
Product group Antibodies
Biorbyt
H2AFX Antibody - N-terminal region (ARP58280_P050)ORB583940
ApplicationsWestern Blot
TargetH2AX
  • SizePrice
Product group Proteins / Signaling Molecules
LifeSpan BioSciences
Human H2AFX / H2AX Protein (Recombinant GST) (Full Length)LS-G28783
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Figure 1. Western blot analysis of HistoneH2A.X using anti-HistoneH2A.X antibody (MP00241). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HistoneH2A.X antigen affinity purified monoclonal antibody (Catalog # MP00241) at 1:5000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HistoneH2A.X at approximately 15 kDa. The expected band size for HistoneH2A.X is at 15 kDa.
Product group Antibodies
References
Boster Bio
Anti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal AntibodyMP00241
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetH2AX
  • SizePrice
Product group Proteins / Signaling Molecules
Biorbyt
Human H2AFX ProteinORB1478151
Protein IDP16104
  • SizePrice
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