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Search results: H2AFX

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Western Blot: H2AX Antibody [TA337175] - Analysis of MOLT4 lysate in the 1) absence and 2) presence of immunizing peptide using Histone H2A.x antibody at 4 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for
Product group Antibodies
OriGene
Histone H2A.X (H2AFX) Rabbit Polyclonal AntibodyTA337175
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetH2AX
  • SizePrice
WB was performed on histone extracts (15 ug) from untreated U2OS cells (lane 1) or from U2OS cells treated with camptothecin (lane 2), and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H2A.XS139p. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.
Product group Antibodies
OriGene
Histone H2A.X (H2AFX) Rabbit Polyclonal AntibodyTA347131
ApplicationsDot Blot, Western Blot, ELISA
ReactivityHuman
TargetH2AX
  • SizePrice
Western blot analysis of extracts of various cell lines, using Histone H2AX antibody (TA377041) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 10s.
Product group Antibodies
OriGene
Histone H2A.X (H2AFX) Rabbit Polyclonal AntibodyTA377041
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat, Other Species
TargetH2AX
  • SizePrice
Western blot analysis of extracts of various cell lines, using Histone H2AX antibody (TA377047) at 1:1000 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 90s.
Product group Antibodies
OriGene
Histone H2A.X (H2AFX) Rabbit Polyclonal AntibodyTA377047
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat, Other Species
TargetH2AX
  • SizePrice
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H2AX-S139 antibody (TA377049) at 1:500 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 180s.
Product group Antibodies
OriGene
Histone H2A.X (H2AFX) Rabbit Polyclonal AntibodyTA377049
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetH2AX
  • SizePrice
Western blot (WB) analysis of Histone H2A.X (Ab-139) polyclonal antibody at 1:5000 dilution Lane1:BV2 whole cell lysate(40ug) Lane2:C6 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug)
Product group Antibodies
OriGene
Histone H2A.X (H2AFX) Rabbit Polyclonal AntibodyTA392546
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetH2AX
  • SizePrice
Western blot (WB) analysis of Histone H2A.X (Phospho-Ser139) polyclonal antibody at 1:1000 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HEK293T treated with UV for 5 minutes then repair for 4 hours whole cell lysate(40ug) Lane3:HEK293T treated with UV for 5 minutes then repair for 6 hours whole cell lysate(40ug)
Product group Antibodies
OriGene
Histone H2A.X (H2AFX) Rabbit Polyclonal AntibodyTA392569
ApplicationsWestern Blot
ReactivityHuman
TargetH2AX
  • SizePrice
Western blot analysis of Histone H2A.X in Raji lysates using Histone H2A.X antibody.
Product group Antibodies
OriGene
Histone H2A.X (H2AFX) Rabbit Polyclonal AntibodyTA384363
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetH2AX
  • SizePrice
Western Blot of Rabbit Anti-H2AX pS139 Antibody. Lane 1: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 2: HEK293T Whole cell Lysate (p/n W09-001-GX5) [+]. Lane 3: MCF-7 Whole cell Lysate (p/n W09-000-360) [+]. Lane 4: U-87-MG Whole cell Lysate (p/n W09-001-GX2) [-]. Primary Antibody: Anti-H2AXpS139 at 5microg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: Universal Fluorescent Buffer (p/n MB-070) for 1hr at RT. Predicted MW: ~15kDa. Observed MW: ~17kDa. Exposure: 10sec.
Product group Antibodies
OriGene
Histone H2A.X (H2AFX) Rabbit Polyclonal AntibodyTA397462S
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetH2AX
  • SizePrice
Western Blot of Rabbit anti-H2AXpS139 antibody. Lane 1: HeLa Lysate stimulated with adriamycin (24 hr). Lane 2: HeLa Lysate unstimulated. Load: 35 ug per lane. Primary antibody: H2AXpS139 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 15.1 kDa, ~18 kDa for H2AXpS139. Other band (s): none.
Product group Antibodies
OriGene
Histone H2A.X (H2AFX) Rabbit Polyclonal AntibodyTA319154
ApplicationsWestern Blot
ReactivityHuman
TargetH2AX
  • SizePrice
Western blot analysis of extracts from HT-29 cells, using Histone H2AX antibody.The lane on the right is treated with the synthesized peptide.
Product group Antibodies
OriGene
Histone H2A.X (H2AFX) Rabbit Polyclonal AntibodyTA311953
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman
TargetH2AX
  • SizePrice
Western blot analysis of Histone H2A.X expression in UV treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
Histone H2A.X (H2AFX) Rabbit Polyclonal AntibodyTA325504
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetH2AX
  • SizePrice
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