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Search results: IL11

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Figure 1. Western blot analysis of IL11 using anti-IL11 antibody (A01160-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL11 antigen affinity purified polyclonal antibody (Catalog # A01160-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL11 at approximately 21KD. The expected band size for IL11 is at 21KD.
Product group Antibodies
Boster Bio
Anti-IL11 Antibody Picoband(r)A01160-1-HRP
ApplicationsWestern Blot, ELISA
TargetIl11
  • SizePrice
Figure 1. Western blot analysis of IL11 using anti-IL11 antibody (A01160-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL11 antigen affinity purified polyclonal antibody (Catalog # A01160-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL11 at approximately 21KD. The expected band size for IL11 is at 21KD.
Product group Antibodies
Boster Bio
Anti-IL11 Antibody Picoband(r)A01160-1-IFLUOR647
ApplicationsWestern Blot, ELISA
TargetIl11
  • SizePrice
Figure 1. Western blot analysis of IL11 using anti-IL11 antibody (A01160-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL11 antigen affinity purified polyclonal antibody (Catalog # A01160-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL11 at approximately 21KD. The expected band size for IL11 is at 21KD.
Product group Antibodies
Boster Bio
Anti-IL11 Antibody Picoband(r)A01160-1-PE
ApplicationsWestern Blot, ELISA
TargetIl11
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL11 AntibodyLS-C812653
ApplicationsWestern Blot, ELISA
TargetIL11
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL11 AntibodyLS-C104366
ApplicationsWestern Blot, ELISA
TargetIL11
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL11 AntibodyLS-C104441
ApplicationsWestern Blot, ELISA
TargetIL11
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL11 AntibodyLS-C130249
ApplicationsELISA
TargetIL11
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL11 AntibodyLS-C37018
ApplicationsFunctional Assay, ELISA, Neutralisation/Blocking
TargetIL11
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL11 AntibodyLS-C662070
ApplicationsWestern Blot, ELISA
TargetIL11
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL11 AntibodyLS-C801328
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetIL11
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL11 AntibodyLS-C804120
ApplicationsWestern Blot, ELISA
TargetIL11
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
IL11 AntibodyLS-C487287
ApplicationsELISA
TargetIL11
  • SizePrice
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