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Search results: MAP22K3 MEK3 MKK3

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Flow cytometric analysis of HeLa cells with MEK3 (4C1) Monoclonal Antibody (bsm-52658R) at a 1:50 dilution (red) compared with an unlabeled control (cells without incubation with primary antibody; black).
Product group Antibodies
Bioss
MEK3 (4C1) Monoclonal AntibodyBSM-52658R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetMAP2K3
  • SizePrice
Mouse muscle lysates probed with MKK3(Ser218) + MKK6(Ser207) Polyclonal Antibody, Unconjugated (bs-3274R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
Bioss
Phospho-MKK3(Ser218) + MKK6(Ser207) Polyclonal AntibodyBS-3274R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMAP2K3
  • SizePrice
Product group Antibodies
Bioss
phospho-MEK3 (Thr222) Polyclonal AntibodyBS-4123R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MEK3 using anti-MEK3 antibody (PB9763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK3 antigen affinity purified polyclonal antibody (Catalog # PB9763) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK3 at approximately 40 kDa. The expected band size for MEK3 is at 40 kDa.
Product group Antibodies
References
Boster Bio
Anti-MEK3/MAP2K3 Antibody Picoband(r)PB9763
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetMAP2K3
  • SizePrice
Figure 2. IP validation of MAP2K6 using Anti-MKK3/6 MAP2K6 Monoclonal Antibody (M02011-2). Immunoprecipitation analysis of Hela cell lysates using MKK3/6 mouse mAb.
Product group Antibodies
Boster Bio
Anti-MKK3/6 MAP2K6 Monoclonal AntibodyM02011-2
ApplicationsImmunoPrecipitation, Western Blot
TargetMAP2K6
  • SizePrice
Boster Kit Box
Product group Assays
Boster Bio
MKK3 Colorimetric Cell-Based ELISA KitEKC1371
  • SizePrice
Anti-MEK3 + MMK6 Antibody [ARC2356]
Product group Antibodies
Antibodies.com
Anti-MEK3 + MMK6 Antibody [ARC2356]A306478
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
MedChem Express
MEK3+MEK6 Antibody (YA3323, Rabbit)HY-P83578
TargetMAP2K3
  • SizePrice
Product group Antibodies
Bioss
MEK3 Polyclonal Antibody, APC ConjugatedBS-1689R-APC
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMAP2K3
  • SizePrice
Product group Antibodies
Bioss
MEK3 Polyclonal Antibody, RBITC ConjugatedBS-1689R-RBITC
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMAP2K3
  • SizePrice
Western blot analysis of MEK3 expression in HepG2 cell lysate.
Product group Antibodies
Boster Bio
Anti-MEK3 MAP2K3 Rabbit Monoclonal AntibodyM02916-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetMAP2K3
  • SizePrice
Product group Proteins / Signaling Molecules
SignalChem
Recombinant Human MEK3/MAP2K3 Protein (Full-length, His Tag), ActiveM04-10H
  • SizePrice
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