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Search results: MAP22K3 MEK3 MKK3

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Western blot analysis of MEK3 expression in HepG2 cell lysate.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K3
  • SizePrice
Anti-MEK3 antibody, PA1377-1, All Western blotting All lanes: Anti-MAP2K3(PA1377-1) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: Rat Kidney Tissue Lysate at 40ug Lane 3: Rat Spleen Tissue Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Lane 5: A549 Whole Cell Lysate at 40ug Lane 6: JURKAT Whole Cell Lysate at 40ug Lane 7: CEM Whole Cell Lysate at 40ug Predicted bind size: 39KD Observed bind size: 39KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Anti-MEK3 antibody, PA1377-1, All Western blotting All lanes: Anti-MAP2K3(PA1377-1) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: Rat Kidney Tissue Lysate at 40ug Lane 3: Rat Spleen Tissue Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Lane 5: A549 Whole Cell Lysate at 40ug Lane 6: JURKAT Whole Cell Lysate at 40ug Lane 7: CEM Whole Cell Lysate at 40ug Predicted bind size: 39KD Observed bind size: 39KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Anti-MEK3 antibody, PA1377-1, All Western blotting All lanes: Anti-MAP2K3(PA1377-1) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: Rat Kidney Tissue Lysate at 40ug Lane 3: Rat Spleen Tissue Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Lane 5: A549 Whole Cell Lysate at 40ug Lane 6: JURKAT Whole Cell Lysate at 40ug Lane 7: CEM Whole Cell Lysate at 40ug Predicted bind size: 39KD Observed bind size: 39KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Anti-MEK3 antibody, PA1377-1, All Western blotting All lanes: Anti-MAP2K3(PA1377-1) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: Rat Kidney Tissue Lysate at 40ug Lane 3: Rat Spleen Tissue Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Lane 5: A549 Whole Cell Lysate at 40ug Lane 6: JURKAT Whole Cell Lysate at 40ug Lane 7: CEM Whole Cell Lysate at 40ug Predicted bind size: 39KD Observed bind size: 39KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Anti-MEK3 antibody, PA1377-1, All Western blotting All lanes: Anti-MAP2K3(PA1377-1) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: Rat Kidney Tissue Lysate at 40ug Lane 3: Rat Spleen Tissue Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Lane 5: A549 Whole Cell Lysate at 40ug Lane 6: JURKAT Whole Cell Lysate at 40ug Lane 7: CEM Whole Cell Lysate at 40ug Predicted bind size: 39KD Observed bind size: 39KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Anti-MEK3 antibody, PA1377-1, All Western blotting All lanes: Anti-MAP2K3(PA1377-1) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: Rat Kidney Tissue Lysate at 40ug Lane 3: Rat Spleen Tissue Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Lane 5: A549 Whole Cell Lysate at 40ug Lane 6: JURKAT Whole Cell Lysate at 40ug Lane 7: CEM Whole Cell Lysate at 40ug Predicted bind size: 39KD Observed bind size: 39KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Anti-MEK3 antibody, PA1377-1, All Western blotting All lanes: Anti-MAP2K3(PA1377-1) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: Rat Kidney Tissue Lysate at 40ug Lane 3: Rat Spleen Tissue Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Lane 5: A549 Whole Cell Lysate at 40ug Lane 6: JURKAT Whole Cell Lysate at 40ug Lane 7: CEM Whole Cell Lysate at 40ug Predicted bind size: 39KD Observed bind size: 39KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Anti-MEK3 antibody, PA1377-1, All Western blotting All lanes: Anti-MAP2K3(PA1377-1) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: Rat Kidney Tissue Lysate at 40ug Lane 3: Rat Spleen Tissue Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Lane 5: A549 Whole Cell Lysate at 40ug Lane 6: JURKAT Whole Cell Lysate at 40ug Lane 7: CEM Whole Cell Lysate at 40ug Predicted bind size: 39KD Observed bind size: 39KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Anti-MEK3 antibody, PA1377-1, All Western blotting All lanes: Anti-MAP2K3(PA1377-1) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: Rat Kidney Tissue Lysate at 40ug Lane 3: Rat Spleen Tissue Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Lane 5: A549 Whole Cell Lysate at 40ug Lane 6: JURKAT Whole Cell Lysate at 40ug Lane 7: CEM Whole Cell Lysate at 40ug Predicted bind size: 39KD Observed bind size: 39KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Anti-MEK3 antibody, PA1377-1, All Western blotting All lanes: Anti-MAP2K3(PA1377-1) at 0.5ug/ml Lane 1: Rat Skeletal Muscle Tissue Lysate at 40ug Lane 2: Rat Kidney Tissue Lysate at 40ug Lane 3: Rat Spleen Tissue Lysate at 40ug Lane 4: HELA Whole Cell Lysate at 40ug Lane 5: A549 Whole Cell Lysate at 40ug Lane 6: JURKAT Whole Cell Lysate at 40ug Lane 7: CEM Whole Cell Lysate at 40ug Predicted bind size: 39KD Observed bind size: 39KD
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityHamster, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice
Figure 1. Western blot analysis of MEK3 using anti-MEK3 antibody (PB9763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK3 antigen affinity purified polyclonal antibody (Catalog # PB9763) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK3 at approximately 40 kDa. The expected band size for MEK3 is at 40 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityBovine, Human, Mouse, Rat
TargetMAP2K3
  • SizePrice