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Search results: MAP2K1 MEK1

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Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S298 antibody (TA378244) at 1:1000 dilution or MAP2K1 antibody (A12687). HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight or treated by ATP(5 mM) at 30°C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of extracts of C6 cells, using Phospho-MEK1-pS221 antibody (TA378245) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of extracts of various cell lines, using Phospho-MEK1-T286 antibody (TA378246) at 1:2000 dilution or MEK1 antibody (A12687). HeLa cells were treated by nocodazole (50 ng/mL) at 37°C for 20 hours or treated by ATP(5 mM) at 30°C for 1 hour. NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37°C for 20 hours or treated by ATP(5 mM) at 30°C for 1 hour. PC-12 cells were treated by Nocodazole (1 microg/mL) at 37°C for 16 hours or treated by ATP(5 mM) at 30°C for 1 hour. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 30s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of extracts of HeLa cells, using Phospho-MEK1-T292 antibody (TA378247) at 1:1000 dilution.HeLa cells were treated by Nocodazole (100 ng/ml) at 37°C for 16 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 60s.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetMAP2K1
  • SizePrice
Western blot of human T47D cells showing specific immunolabeling of the ~45k MEK 1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase: I>> Ptase) The blot is identical to the control except that it was incubated in I>> Ptase (1200 units for 30 min) before being exposed to the MEK 1 Thr386 antibody. The immunolabeling of MEK 1 is completely eliminated by I>> Ptase.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK1 phosphorylation expression in K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK1/2 phosphorylation expression in PMA treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK1/2 phosphorylation expression in UV treated Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
WB: The whole cell lysates derived from 3T3 were immunoblotted by Rabbit anti-MEK1/2 antibody at 1:1000.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot (WB) analyzes of MEK1/2 antibody (Cat.-No.: AP20701PU-N) in extracts from hela and raw264.7 cells.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analyzes of MEK1/2 antibody (Cat.-No.: AP20702PU-N) in extracts from HT-29 cells.
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot of human T47D cells showing specific immunolabeling of the ~45 kDa MEK 1 protein phosphorylated at Thr386 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
ReactivityBovine, Canine, Chicken, Human, Mouse, Primate, Rat, Xenopus
TargetMAP2K1
  • SizePrice