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Search results: MAP2K1 MEK1

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Western blot analysis of extracts of various cell lines, using MAP2K1/MAP2K2 antibody (TA378248) at 1:500 dilution. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 60s.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA378248
ApplicationsWestern Blot
ReactivityMouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of extracts of various mouse tissues, using MAP2K1 antibody.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA326799
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of extracts of various cell lines, using Phospho-MAP2K1-S298 antibody (TA378244) at 1:1000 dilution or MAP2K1 antibody (A12687). HeLa cells were treated by PMA/TPA (200 nM) at 37°C for 15 minutes after serum-starvation overnight or treated by ATP(5 mM) at 30°C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA378244
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of extracts of C6 cells, using Phospho-MEK1-pS221 antibody (TA378245) at 1:1000 dilution.C6 cells were treated by PMA/TPA (200 nM) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA378245
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of extracts of various cell lines, using Phospho-MEK1-T286 antibody (TA378246) at 1:2000 dilution or MEK1 antibody (A12687). HeLa cells were treated by nocodazole (50 ng/mL) at 37°C for 20 hours or treated by ATP(5 mM) at 30°C for 1 hour. NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37°C for 20 hours or treated by ATP(5 mM) at 30°C for 1 hour. PC-12 cells were treated by Nocodazole (1 microg/mL) at 37°C for 16 hours or treated by ATP(5 mM) at 30°C for 1 hour. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% BSA. - Detection: ECL Basic Kit . - Exposure time: 30s.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA378246
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of extracts of HeLa cells, using Phospho-MEK1-T292 antibody (TA378247) at 1:1000 dilution.HeLa cells were treated by Nocodazole (100 ng/ml) at 37°C for 16 hours. - Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. - Lysates/proteins: 25ug per lane. - Blocking buffer: 3% nonfat dry milk in TBST. - Detection: ECL Basic Kit . - Exposure time: 60s.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA378247
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK1 phosphorylation expression in K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA325635
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK1/2 phosphorylation expression in PMA treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA325636
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot analysis of MEK1/2 phosphorylation expression in UV treated Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA325637
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot of human T47D cells showing specific immunolabeling of the ~45k MEK 1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase: I>> Ptase) The blot is identical to the control except that it was incubated in I>> Ptase (1200 units for 30 min) before being exposed to the MEK 1 Thr386 antibody. The immunolabeling of MEK 1 is completely eliminated by I>> Ptase.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA309168
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetMAP2K1
  • SizePrice
WB: The whole cell lysates derived from 3T3 were immunoblotted by Rabbit anti-MEK1/2 antibody at 1:1000.
Product group Antibodies
OriGene
MEK1 (MAP2K1) Rabbit Polyclonal AntibodyTA354399
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
Western blot (WB) analyzes of MEK1/2 antibody (Cat.-No.: AP20701PU-N) in extracts from hela and raw264.7 cells.
Product group Antibodies
OriGene
MEK1 (MAP2K1) (+MAP2K2) Rabbit Polyclonal AntibodyAP20701PU-N
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetMAP2K1
  • SizePrice
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