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Search results: MCU

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Western blot analysis of MCUR1 using anti-MCUR1 antibody (A08547-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. Lane 2: human MDA-MB-231 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human MDA-MB-453 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: rat spleen tissue lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse Ana-1 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCUR1 antigen affinity purified polyclonal antibody (Catalog # A08547-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCUR1 at approximately 40KD. The expected band size for MCUR1 is at 40KD.
Product group Antibodies
Boster Bio
Product group Antibodies
Arigo Biolaboratories
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetMCUR1
  • SizePrice
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
Cusabio
ReactivityHuman
  • SizePrice
Product group Antibodies
Cusabio
ApplicationsELISA
ReactivityHuman
  • SizePrice
WB Suggested Anti-CCDC90A Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human brain
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
TargetMCUR1
  • SizePrice