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Figure 1. Western blot analysis of ODF2L using anti-ODF2L antibody (A16444-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF2L antigen affinity purified polyclonal antibody (Catalog # A16444-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODF2L at approximately 65 kDa. The expected band size for ODF2L is at 74 kDa.
Product group Antibodies
Boster Bio
Anti-ODF2L Antibody Picoband(r)A16444-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetODF2L
  • SizePrice
Figure 1. Western blot analysis of ODF2L using anti-ODF2L antibody (A16444-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF2L antigen affinity purified polyclonal antibody (Catalog # A16444-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODF2L at approximately 65 kDa. The expected band size for ODF2L is at 74 kDa.
Product group Antibodies
Boster Bio
Anti-ODF2L Antibody Picoband(r)A16444-1
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetODF2L
  • SizePrice
Figure 1. Western blot analysis of ODF2L using anti-ODF2L antibody (A16444-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF2L antigen affinity purified polyclonal antibody (Catalog # A16444-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODF2L at approximately 65 kDa. The expected band size for ODF2L is at 74 kDa.
Product group Antibodies
Boster Bio
Anti-ODF2L Antibody Picoband(r)A16444-1-PE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetODF2L
  • SizePrice
Figure 1. Western blot analysis of ODF2L using anti-ODF2L antibody (A16444-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF2L antigen affinity purified polyclonal antibody (Catalog # A16444-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODF2L at approximately 65 kDa. The expected band size for ODF2L is at 74 kDa.
Product group Antibodies
Boster Bio
Anti-ODF2L Antibody Picoband(r)A16444-1-FITC
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetODF2L
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ODF2L AntibodyLS-C803533
ApplicationsWestern Blot, ELISA
TargetODF2L
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ODF2L AntibodyLS-C753514
ApplicationsWestern Blot, ELISA
TargetODF2L
  • SizePrice
Product group Antibodies
MyBioSource
ODF2L, CT (ODF2L, KIAA1229, Outer dense fiber protein 2-like) (MaxLight 405)MBS6327617
  • SizePrice
Product group Antibodies
MyBioSource
ODF2L, CT (ODF2L, KIAA1229, Outer dense fiber protein 2-like) (MaxLight 550)MBS6327619
  • SizePrice
Product group Antibodies
MyBioSource
ODF2L, CT (ODF2L, KIAA1229, Outer dense fiber protein 2-like) (Azide free) (HRP)MBS6327616
  • SizePrice
Product group Antibodies
MyBioSource
ODF2L, CT (ODF2L, KIAA1229, Outer dense fiber protein 2-like) (MaxLight 650)MBS6327620
  • SizePrice
Product group Antibodies
MyBioSource
ODF2L, CT (ODF2L, KIAA1229, Outer dense fiber protein 2-like) (MaxLight 750)MBS6327621
  • SizePrice
Product group Antibodies
MyBioSource
ODF2L, CT (ODF2L, KIAA1229, Outer dense fiber protein 2-like) (MaxLight 490)MBS6327618
  • SizePrice
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