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Figure 1. Western blot analysis of ODF2L using anti-ODF2L antibody (A16444-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF2L antigen affinity purified polyclonal antibody (Catalog # A16444-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODF2L at approximately 65 kDa. The expected band size for ODF2L is at 74 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
TargetODF2L
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ODF2L AntibodyLS-C753514
ApplicationsWestern Blot, ELISA
TargetODF2L
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ODF2L AntibodyLS-C803533
ApplicationsWestern Blot, ELISA
TargetODF2L
  • SizePrice
Product group Molecular Biology
Abbexa
  • SizePrice
Product group Molecular Biology
Abbexa
  • SizePrice
Product group Antibodies
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetODF2
  • SizePrice