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Search results: ORM1

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Figure 1. Western blot analysis of ORM1 using anti-ORM1 antibody (PB9956). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORM1 antigen affinity purified polyclonal antibody (Catalog # PB9956) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORM1 at approximately 40-47 kDa. The expected band size for ORM1 is at 23 kDa.
Product group Antibodies
Boster Bio
Anti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband(r)PB9956-FITC
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetORM1
  • SizePrice
Figure 1. Western blot analysis of ORM1 using anti-ORM1 antibody (PB9956). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORM1 antigen affinity purified polyclonal antibody (Catalog # PB9956) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORM1 at approximately 40-47 kDa. The expected band size for ORM1 is at 23 kDa.
Product group Antibodies
Boster Bio
Anti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband(r)PB9956-HRP
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetORM1
  • SizePrice
Figure 1. Western blot analysis of ORM1 using anti-ORM1 antibody (PB9956). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORM1 antigen affinity purified polyclonal antibody (Catalog # PB9956) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORM1 at approximately 40-47 kDa. The expected band size for ORM1 is at 23 kDa.
Product group Antibodies
Boster Bio
Anti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband(r)PB9956-IFLUOR647
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetORM1
  • SizePrice
Figure 1. Western blot analysis of ORM1 using anti-ORM1 antibody (PB9956). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORM1 antigen affinity purified polyclonal antibody (Catalog # PB9956) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORM1 at approximately 40-47 kDa. The expected band size for ORM1 is at 23 kDa.
Product group Antibodies
Boster Bio
Anti-Alpha 1 Acid Glycoprotein/ORM1 Antibody Picoband(r)PB9956-PE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetORM1
  • SizePrice
Product group Assays
Abbexa
Cow Alpha-1-Acid Glycoprotein 1 (ORM1) ELISA KitABX150056
  • SizePrice
Product group Assays
Abbexa
Human Alpha-1-Acid Glycoprotein 1 (ORM1) ELISA KitABX150486
  • SizePrice
Product group Assays
Abbexa
Mouse Alpha-1-Acid Glycoprotein 1 (ORM1) ELISA KitABX153574
  • SizePrice
Product group Assays
Abbexa
Pig Alpha-1-Acid Glycoprotein 1 (ORM1) ELISA KitABX154876
  • SizePrice
Product group Assays
Abbexa
Rat Alpha-1-Acid Glycoprotein 1 (ORM1) ELISA KitABX156921
  • SizePrice
Product group Assays
Abbexa
Human Alpha-1-Acid Glycoprotein 1 (ORM1) CLIA KitABX195067
  • SizePrice
Product group Assays
Abbexa
Rat Alpha-1-Acid Glycoprotein 1 (ORM1) CLIA KitABX195068
  • SizePrice
Product group Assays
Abbexa
Mouse Alpha-1-Acid Glycoprotein 1 (ORM1) CLIA KitABX195069
  • SizePrice
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