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Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (A00871). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (Catalog # A00871) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 11KD. The expected band size for PF4 is at 11KD.
Product group Antibodies
Boster Bio
Anti-PF4 Antibody Picoband(r)A00871-DYLIGHT488
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetPf4
  • SizePrice
Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (A00871). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (Catalog # A00871) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 11KD. The expected band size for PF4 is at 11KD.
Product group Antibodies
Boster Bio
Anti-PF4 Antibody Picoband(r)A00871-DYLIGHT550
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetPf4
  • SizePrice
Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (A00871). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (Catalog # A00871) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 11KD. The expected band size for PF4 is at 11KD.
Product group Antibodies
Boster Bio
Anti-PF4 Antibody Picoband(r)A00871-DYLIGHT594
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetPf4
  • SizePrice
Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (A00871). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (Catalog # A00871) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 11KD. The expected band size for PF4 is at 11KD.
Product group Antibodies
Boster Bio
Anti-PF4 Antibody Picoband(r)A00871-FITC
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetPf4
  • SizePrice
Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (A00871). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (Catalog # A00871) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 11KD. The expected band size for PF4 is at 11KD.
Product group Antibodies
Boster Bio
Anti-PF4 Antibody Picoband(r)A00871-HRP
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetPf4
  • SizePrice
Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (A00871). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (Catalog # A00871) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 11KD. The expected band size for PF4 is at 11KD.
Product group Antibodies
Boster Bio
Anti-PF4 Antibody Picoband(r)A00871-IFLUOR647
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetPf4
  • SizePrice
Figure 1. Western blot analysis of PF4 using anti-PF4 antibody (A00871). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PF4 antigen affinity purified polyclonal antibody (Catalog # A00871) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PF4 at approximately 11KD. The expected band size for PF4 is at 11KD.
Product group Antibodies
Boster Bio
Anti-PF4 Antibody Picoband(r)A00871-PE
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetPf4
  • SizePrice
Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
Recombinant Platelet Factor 4 (PF4)RPA172HU01
ApplicationsWestern Blot, Other Application
Protein IDP02776
  • SizePrice
Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
Recombinant Platelet Factor 4 (PF4)RPA172MU01
ApplicationsWestern Blot, Other Application
Protein IDQ9Z126
  • SizePrice
Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
Recombinant Platelet Factor 4 (PF4)RPA172MU02
ApplicationsWestern Blot, Other Application
Protein IDQ9Z126
  • SizePrice
Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
Recombinant Platelet Factor 4 (PF4)RPA172RA01
ApplicationsWestern Blot, Other Application
Protein IDP06765
  • SizePrice
Product group Proteins / Signaling Molecules
Cloud-Clone Corp.
Recombinant Platelet Factor 4 (PF4)RPA172RA02
ApplicationsWestern Blot, Other Application
Protein IDP06765
  • SizePrice
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