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Western blot analysis of PCSK4 using anti-PCSK4 antibody (PA2086). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCSK4 antigen affinity purified polyclonal antibody (Catalog # PA2086) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCSK4 at approximately 83KD. The expected band size for PCSK4 is at 83KD.
Product group Proteins / Signaling Molecules
Boster Bio
Anti-ProProtein Convertase PC4 AntibodyPA2086
Protein IDP29121
Product group Antibodies
LifeSpan BioSciences
F2 / Prothrombin / Thrombin Antibody (clone Ca-Pro 20)LS-C348202
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetF2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PDYN / ProDynorphin Antibody (Preservative Free, Biotin)LS-C743790
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PDYN / ProDynorphin Antibody (aa1-13, Serum)LS-C42744
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Frozen, RadioImmunoAssay
ReactivityPorcine
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PDYN / ProDynorphin Antibody (aa1-17, Serum)LS-C42740
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, RadioImmunoAssay
ReactivityHuman
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PDYN / ProDynorphin Antibody (aa143-192, Biotin)LS-C463852
ApplicationsWestern Blot
ReactivityBovine, Canine, Chicken, Equine, Guinea Pig, Goat, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Zebra Fish
TargetPDYN
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PDYN / ProDynorphin Antibody (aa143-192, FITC)LS-C463853
ApplicationsWestern Blot
ReactivityBovine, Canine, Chicken, Equine, Guinea Pig, Goat, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Zebra Fish
TargetPDYN
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PDYN / ProDynorphin Antibody (aa143-192, HRP)LS-C463854
ApplicationsWestern Blot
ReactivityBovine, Canine, Chicken, Equine, Guinea Pig, Goat, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Primate, Rabbit, Rat, Zebra Fish
TargetPDYN
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PDHX / Protein X / ProX Antibody (aa220-468, Cy3)LS-C704902
ApplicationsWestern Blot
ReactivityHuman, Porcine
TargetPDHX
  • SizePrice
More sizes
Product group Antibodies
LifeSpan BioSciences
PDHX / Protein X / ProX Antibody (aa222-471, Cy3)LS-C704903
ApplicationsWestern Blot
ReactivityMouse
TargetPDHX
  • SizePrice
More sizes
Product group Antibodies
LifeSpan BioSciences
PDHX / Protein X / ProX Antibody (aa222-471, Biotin)LS-C688971
ApplicationsWestern Blot
ReactivityMouse
TargetPDHX
  • SizePrice
More sizes
Product group Antibodies
LifeSpan BioSciences
PDHX / Protein X / ProX Antibody (aa220-468, Biotin)LS-C688972
ApplicationsWestern Blot
ReactivityHuman, Porcine
TargetPDHX
  • SizePrice
More sizes
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