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Search results: Siglec-3

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Product group Proteins / Signaling Molecules
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Product group Antibodies
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Product group Proteins / Signaling Molecules
MyBioSource
Protein IDP20138
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Product group Proteins / Signaling Molecules
MyBioSource
Protein IDP20138
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Immunohistochemical staining of human lung shows moderate positivity in macrophages.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetCD33
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Western blot analysis of CD33 expression in THP1 cell lysate using anti-CD33 antibody (M01508). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD33 antigen affinity purified polyclonal antibody (Catalog # M01508) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CD33.
Product group Antibodies
Boster Bio
ApplicationsImmunoPrecipitation, Western Blot
ReactivityHuman
TargetCD33
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Western Blot: CD33 Antibody (44M12D3) [TA336936] - analysis of CD33 in A) a partial recombinant protein and B) HCT-116 lysate using CD33 antibody at 2 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this
Product group Antibodies
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCD33
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