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Search results: anti-Collagen/currency/EUR

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Western blot analysis of Collagen X expression in human fetal skin lysate.
Product group Antibodies
Boster Bio
Immunohistochemical analysis of paraffin-embedded human kidney, using Collagen I Antibody.
Product group Antibodies
Boster Bio
IHC analysis of Collagen using anti-Collagen antibody (PB9099). Collagen was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Collagen Antibody (PB9099) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Product group Antibodies
Boster Bio
Product group Antibodies
Boster Bio
Western blot analysis of Collagen II using anti-Collagen II antibody (A00517-1).
Product group Antibodies
Boster Bio
Western blot analysis of COL17A1 using anti-COL17A1 antibody (A03031-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-COL17A1 antigen affinity purified polyclonal antibody (Catalog # A03031-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for COL17A1 at approximately 125KD. The expected band size for COL17A1 is at 150KD.
Product group Antibodies
Boster Bio
Product group (Cell) Culture
Absolute Antibody
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Product group (Cell) Culture
Absolute Antibody
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Product group (Cell) Culture
Absolute Antibody
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Product group (Cell) Culture
Absolute Antibody
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Product group (Cell) Culture
Absolute Antibody
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Product group (Cell) Culture
Absolute Antibody
  • SizePrice