Secretory Component Antibody (clone NI 194-4 (A89-39), Biotin)

LifeSpan BioSciences

Secretory Component Antibody (clone NI 194-4 (A89-39), Biotin)

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Human Secretory Component. The reactivity of the antiserum is restricted to secretory component as tested in indirect binding immunoblotting and indirect immunoperoxidase staining. Cross-reactivity The antiserum does not react with any other component of

This product is intended for use to identify the presence of free or bound secretory component in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. As a second step an avidin or streptavidin conjugate of the users choice has to be used. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards; in ELISA from 1:25 upwards; in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. ELISA, IF, WB This product is intended for use to identify the presence of free or bound secretory component in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. As a second step an avidin or streptavidin conjugate of the users choice has to be used. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards; in ELISA from 1:25 upwards; in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

ImmunoFluorescence, Western Blot, ELISA

Research Use Only

Monoclonal

NI 194-4 (A89-39)

Biotin

...

Mouse

IgG1

Human

-20°C,2°C to 8°C

12352203