axoCells Human iPSC-Derived Sensory Neuron Media and Supplement Kit contains 1 bottle of 250 mL Sensory Neuron Maintenance Medium and one vial (1ml) of the Maturation Maximizer. Suitable for use with axoCells Sensory Neurons (ax0055, ax0555).Maturation Maximizer is a fully defined medium supplement for enhancing the existing Sensory Neuron Maintenance Medium (ax0060). It works by mimicking in vivo signals between sensory neurons and their supporting cells. The supplement contains signaling factors present in the peripheral nervous system and in particular the native environment of sensory neurons. Utilizing this supplement accelerates the maturation of iPSC-derived sensory neurons in vitro.iPSC-derived sensory neurons treated with the Maximizer express key markers, such as TRPv1, TRPa1, TRPv2, TRPv3 and TRPm8. Kinetic states of e.g. TRPv1 are in a fast response inward rectifying state, to agonists such as Capsaicin. Neuronal responses to capsaicin occur at 1uM for 90% of the sensory neuron population on day 22 of culture.The Maturation Maximizer supplement makes the Human iPSC-derived sensory neuronal culture a more physiological relevant model.
