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Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue, Rat liver tissue All lanes: SEPT11 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 51 kDa Observed band size: 50 kDa
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue, Rat liver tissue All lanes: SEPT11 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 51 kDa Observed band size: 50 kDa
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, Rat brain tissue, Rat liver tissue All lanes: SEPT11 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 51 kDa Observed band size: 50 kDa

SEPT11 Antibody

CSB-PA878895LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetSEPTIN11
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Overview

  • Supplier
    Cusabio
  • Product Name
    SEPT11 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55752
  • Target name
    SEPTIN11
  • Target description
    septin 11
  • Target synonyms
    epididymis secretory sperm binding protein; SEPT11; septin-11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NVA2
  • Protein Name
    Septin-11
  • Scientific Description
    Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in the cytoarchitecture of neurons, including dendritic arborization and dendritic spines, and in GABAergic synaptic connectivity (By similarity). During Listeria monocytogenes infection, not required for the bacterial entry process, but restricts its efficacy.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Septin 11/SEPTIN11 using anti-Septin 11/SEPTIN11 antibody (A12762-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse barin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Septin 11/SEPTIN11 antigen affinity purified polyclonal antibody (Catalog # A12762-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Septin 11/SEPTIN11 at approximately 49 kDa. The expected band size for Septin 11/SEPTIN11 is at 49 kDa.
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