IHC-P analysis of mouse heart tissue using GTX22818 SERCA1 ATPase antibody [IIH11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
![IHC-P analysis of mouse lymph node tissue using GTX22818 SERCA1 ATPase antibody [IIH11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22818/GTX22818_1156_IHC-P_w_23060620_602.webp "IHC-P analysis of mouse lymph node tissue using GTX22818 SERCA1 ATPase antibody [IIH11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of mouse skeletal muscle tissue using GTX22818 SERCA1 ATPase antibody [IIH11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22818/GTX22818_1157_IHC-P_w_23060620_492.webp "IHC-P analysis of mouse skeletal muscle tissue using GTX22818 SERCA1 ATPase antibody [IIH11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![WB analysis of 25 ug of human skeletal muscle (lane 1), rat skeletal muscle (lane 2) and mouse skeletal muscle (lane 3) using GTX22818 SERCA1 ATPase antibody [IIH11]. Dilution : 1:2000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22818/GTX22818_1608_WB_w_23060620_769.webp "WB analysis of 25 ug of human skeletal muscle (lane 1), rat skeletal muscle (lane 2) and mouse skeletal muscle (lane 3) using GTX22818 SERCA1 ATPase antibody [IIH11]. Dilution : 1:2000")
![ICC/IF analysis of C6 cells using GTX22818 SERCA1 ATPase antibody [IIH11]. <Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actinbr>Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22818/GTX22818_504_ICC-IF_w_23060620_632.webp "ICC/IF analysis of C6 cells using GTX22818 SERCA1 ATPase antibody [IIH11]. <Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actinbr>Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![IHC-P analysis of dog skeletal muscle tissue using GTX22818 SERCA1 ATPase antibody [IIH11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX22818/GTX22818_961_IHC-P_w_23060620_453.webp "IHC-P analysis of dog skeletal muscle tissue using GTX22818 SERCA1 ATPase antibody [IIH11].")
IHC-P analysis of mouse heart tissue using GTX22818 SERCA1 ATPase antibody [IIH11]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
SERCA1 ATPase antibody [IIH11]
GTX22818
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Guinea Pig, Human, Mouse, Rabbit, Rat
TargetATP2A1
Overview
- SupplierGeneTex
- Product NameSERCA1 ATPase antibody [IIH11]
- Delivery Days Customer9
- Antibody SpecificityThis antibody detects sarcoplasmic or endoplasmic reticulum calcium 1 (SERCA1) ATPase. It recognizes an epitope between amino acids 199-505 of rabbit skeletal muscle ATPase, a region that is not exposed in native sarcoplasmic reticulum.
- Application Supplier NoteWB: 1:500-1:5000. ICC/IF: 1:500. IHC-Fr: 1:20-200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDIIH11
- ConjugateUnconjugated
- Gene ID100037716
- Target nameATP2A1
- Target descriptionATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1
- Target synonymsATP2A3; ATPase, Ca++ transporting, cardiac muscle, fast twitch 1; ATPase, Ca++ transporting, ubiquitous; calcium pump 1; endoplasmic reticulum class 1/2 Ca(2+) ATPase; sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1; SERCA1a; SR Ca(2+)-ATPase 1
- HostMouse
- IsotypeIgG1
- Protein IDP04191
- Protein NameSarcoplasmic/endoplasmic reticulum calcium ATPase 1
- ReactivityCanine, Guinea Pig, Human, Mouse, Rabbit, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Salinomycin triggers endoplasmic reticulum stress through ATP2A3 upregulation in PC-3 cells. Zhang Y et al., 2019 Apr 25, BMC CancerRead more